The Initial Study Of The Biomarkers In Rats Plasma Of Subchronic Induction Of Acrylamide

ObjectiveAcrylamide (ACR) is a kind of widely-used industrial material. It is regarded as a neurotoxicant and human potential carcinogen through the studies of more than thirty years. But the exact mechanism of action of its neurotoxicity has not been completely clear until now. In this study, we made the laboratory animal model of subchronic intoxication of ACR and detected the changes of levels of cytoskeletal proteins, neurone specific enolase (NSE) and glial fibrillary acidic protein (GFAP) in plasma of rats. It will offer the cues and evidences for exploring the mechanism of ACR neurotoxicity and searching biomarkers.Methods1. Male Wistar rats weighing 180～220g were divided into three groups, each 9 animals. The rats in group 1 served as control, and received normal saline. The animals in group 2 and 3 were given ACR dissolved in physiological saline(20, 40 mg/kg i.p. 3 days/week) for 8 weeks.2. Neurobehavioral index were determined per week. The changes of general status,...