Expression Of Livin, PTEN And MMP-9Inretinoblastoma And Their Clinical Significance

Objective:To study the expression of Livin, PTEN and MMP-9and discuss their clinical significance in retinoblastoma.Methods:The expression of Livin, PTEN and MMP-9in56samples of retinoblastoma tissues and10samples of normal retinal was examined with immunohistochemistry.Results:(1).The positive expression rates of PTEN protein was100%in normal retinal tissue and significantly higher than50%of positive expression in retinoblastoma tissues. The positive expression rates of PTEN protein was correlated with clinical stages, histopathological type and optic nerve infiltration rather than age and gender. Livin protein did not expressed in normal retinal tissue, but exhibited65%positive expression rates in retinoblastoma tissues, in which the difference of both expression rates is significant.(2).The positive expression rates of MMP-9was41.3%, whereas it was not expressed in normal retina tissues, and the difference was significant (p<0.05). The positive expression rates of PTEN, Livin and MMP-9in retinoblastoma tissues were associated with clinical stages, histopathological type and optic nerve infiltration rather than the age and gender of patients.(3).Expression rate of PTEN was negatively correlated to both that of Livin and MMP-9, whereas the latter two were positively correlated in expression rate of retinoblastoma tissues.(4).The expression of PTEN was negatively correlate with the coexpression of MMP-9and Livin.Conclusion:(1).The protein expression levels of Livin and MMP-9was frequently upregulated in retinoblastoma, whereas normal retina tissues show negative. This suggested that NGAL and NGALR may associate with occurrence of colorectal cancer.(2).Overexpression of Livin,PTEN and MMP-9were closely associated with histopathologic grading and metastasis in retinoblastoma.This suggestted that Livin.PTEN及MMP-9may play a key role in development, invasion and metastasis of retinoblastoma.(3).Co-expression of Livin and MMP-9was significantly correlated with histopathology, clinical stage, pathological typing and whether there was optic nerve infiltration in retinoblastoma. This indicated that retinoblastoma with co-expression Livin and MMP-9may be of higher degree of malignancy.(4).Correlation between Livin PTEN and MMP-9expression in retinoblastoma indicated that their expression may exist within a kind of synergy, combined detection of Livin.PTEN and MMP-9will contribute to the clinical diagnosis and efficacy evaluation of retinoblastoma.