Experimental Research On Effect Of Chailing Tiaogan Granule On Expression Of SOCS-3and SREBP-1c MRNA In Liver Tissue Of NAFLD Rats

Background: Non-alcoholic fatty liver disease (NAFLD), aclinical syndrome charactered by hepatocyte fatty degeneration,storage of adipose, and without excessive alcohol use, is relatedto heredity, environment, and metabolic stress. With thedevelopment of economy and improvement of living standard of thepeople, the structure of daily diet varies, and the incidence ofNAFLD, which has been a usual chronic disease harmful to human health,keeps growing year by year. It is regarded that the courses of NAFLDinclude simple steatosis, steatohepatitis, adipose liver fibrosis,and finally lead to cirrhosis and liver cancer. The mechanism ofNAFLD, which has been widely researched, is still unclear. and idealmedicine is still under development. So studying the mechanism ofNAFLD, and developing safe and effective traditional Chinesemedicine method for curing NAFLD has been an important subject.Objective: This subject focuses on the effect of insulinresistance (IR) in NAFDL. By means of experiments, the effect ofChailing Tiaogan Granule on the expression of gene SOCS-3andSREBP-1c in liver tissue of NAFLD rats. The effect and the mechanismfor curing NAFLD of Chailing Tiaogan Granule is discussed andresearched, to provide a scientific basis for future developmentof corresponding proprietary Chinese medicinesMethod: Feed80male Wistar rats for a week, then71are randomlyselected for modeling, and are fed with high-fat food for10weeks.8are killed for confirming the success of modeling, then63arerandomized into7groups with9in each, including the Model Group,the Diet-Controlling Group, the Ganle Group, the Sanqi Fatty LiverBall Comparing Gruop (SFLB Group), the Chailing Tiaogan GranuleHigh/Medium/Low Dosage Groups, while the9rats not for modelingare arranged into the Normal Group. After the medication, compare the histopathological symptom before and after treatment of eachgroup, measure the levels of ALT, AST, TC TG FPG, and FINS in serum;observe the m-RNA gene expression of the liver SOCS-3and SREBP-1cof the rats with the method of RT-PCR.Results:Chailing Tiaogan Granule can lower the body and liverweight indexes of the rats for experiment, lower the content of ALT,AST, TC TG FPG,and FINS in serum. It can reduce the gene expressionof SOCS-3mRNA&SREBP-1cmRNA, and alleviate the symptoms of thetissue of the rats for experiments histopathologically, and finallyreturn to normal.Conclusion:NAFLD and IR are not independent, they can beconnected by a series of factors. By regulating the gene expessionof SOCS-3&SREBP-1c, Chailing Tiaogan Granule can lower the bodyweight and liver weight index of experimental rat, effectively lowerALT，AST，TC, TG，FPG，FINS in serum, and help the histopathologicalsymptom of tissue of rats and return to normal. In conclusion,Chailing Tiaogan Granule is effective to NAFLD and worthy of furtherresearch,...