The Screening And Preparation Technolgy Study Of Daphne Giraldii Nitsch’s Effective Components To Treat RA

Daphne giraldii Nitsch, a famous folk medicine wildly used in west China for treating rheumatism disease clinically, is the bark of Thymelaeaceae Daphne giraldii Nitsche. As the western region folk medicine, it is mainly used for treating rheumatism disease clinically. Integrated predecessor’s research results showed that, the total coumarin composition is the effective anti-inflammatory analgesia components, One of daphnetin for its major components, diferpenoid ortho ester composition is the stimulating component that produce skin irritation and so on side effects. Based on the above research background, we believe that the total coumarin composition is not the Daphne giraldii Nitsch’s only effective component that anti-rheumatoid arthritis, the saponins ruom planta maybe one of the effective component that anti-rheumatoid arthritis. According to these, we start the screening and preparation technology study of Daphnegiraldii Nitsch’s effective components to treat RA. For the fully developed of Daphne giraldii Nitsch medicinal resources, proved its material basis for efficacy and the composition of irritation and toxicity, in order to preparing a more safe and efficient treatment of RA drugs. On the basis of laboratory pilot study of preliminary work, this thesis guided by biological activity, finally identified total conmarins and total saponine as Daphne giraldii Nitsch’s active ingredient for the treatment of RA. In the fundamentally of many experiments, the research man Isolated the active ingrediend that Daphne giraldii Nitsch antagonism rheumatoid arthritis, and to determine the activity of each part, corroborated the strong acrimony of diterpenoid ortho ester composition, the total coumarin composition and the saponins are The Daphne giraldii Nitsch’s major effective component group that anti-rheumatoid arthritis, excepted the toxic diterpenoid ortho ester composition, and determined preparation process of coumarin and total saponins that have active ingredient.1 Literature studyConsult the relevant literature stury of rheumatoid arthritis, Daphne giraldii Nitsch and the other plants of the genus gnidimacrin, understand the overview in the current treatment of rheumatoid arthritis, familiar with the recent chemical constituents and biological activity research of Daphne giraldii Nitsch and the other plants that belong to the same plant family, lay the theoretical foundation to the latter experimental study.2 To screen Daphne giraldii Nitsch effective components treatment of RA . At home and abroad, lack of systemic toxicity studies to Daphne giraldii Nitsch anti-RA’s pharmacological effects and irritating, lack of an effective theory for clinical application. According to this, for the total extract and its components such as petroleum ether part, ethyl acetate extract part, n-butanol part, water residual mother liquor, conducted the following experiment: (1) xylene-induced ear swelling in mice;(2) Cotton granuloma experiment in mice;(3) acetic acid-induced writhing response in mice;(4) Rats adjuvants arthritis experiment;(5)Rabbit skin irritation.The results showed that, the main effective compositions of Daphne giraldii Nitsch for treatment of RA are ethyl frations and n-BuOH frations, and the machanism could be inhibit the production of inflammation medium. In addition, the diterpenoid ortho ester in the petroleum ether fratction has strong irritation, which is the major irritation contribution. 3 The preparation process study of total coumarin and total saponins in Daphne giraldii Nitsch.Ultraviolet method was used to determine the content of total coumarin and total saponins in Daphne giraldii Nitsch. HPLC was used to determine the content of three kind of phenylpropanoids in Daphne giraldii, and for the study ways inspection .Result: precision of test methods, stability, ruggedness and average recovery were all consistent with the provisions and the method is simple. Preparation of total Coumarins: successively used macroporous resin adsorption technology, organic solvent extraction and other different methods, finally, organic solvents (ethyl acetate) extraction method used as the extraction process for its simple and Content meet the requirements. Preparation of total saponins: Macroreti- cular resin used for enrichment and purification.