Multilayer Cpo Electrochemical Behavior Of The Modified Electrode And Its Application In Organic Catalytic Reaction

Chlo ro pe ro xid a se (CPO) is a kind of protease which is widely used and has many types of catalytic reaction. This paperused laboratoryis experiencesforthe following research work1. Study on the electrochemical behaviors of different laye rs CPO modified electrodesIn order to enhance the immobilized quantity of enzyme on the modified electrode and improve the stability of the modified electrode, using the experience of multilayer enzyme membrane modified electrode preparation in the biosensor as a reference, the multilayer CPO modified electrode preparation method had been studied, especially the electrochemical performance of two-layer CPO modified electrode. The cyclic voltammetry results showed that the redox current was much larger and the catalytic effect of the oxygen reduction was more significant with the two-layer CPO modified electrode. Ele ctro chemical Impedance Spectroscopy (E(?)S) was applied to further explain the influence of membrane’s structure on electron transport of the modified electrode.2. The comparison of different bio-enzyme modified electrodes c atalytic chlorination of MCDCPO can catalyze monochlorodimedone (MCD) and reaction product is dichlorodimedone (DCD). This reaction process can be controlled by UV-spectroscopy which is simply and convenient. In this paper, used this reaction, the comparision of the activity of catalytic chloiination between Mb, Hb and CPO have been made. The results showed that CPO’s catalytic chlorination capability was strongerthan o the rs.3. The application of Chloroperoxidase (CPO) modified electrodesfororganic catalytic reaction This study attempted to use the (CPO/PLL)2/GC modified electrode to catalyze oxidation of Benzyl methyl sulfide by in situ generated H2O2. The results showed that the target product Phenyl methyl sulfoxide was obtained and the quantity of product increased with the catalytic reaction time. An environment friendly and the green organic synthesis reaction catalyzed by the biologicalenzyme wasdeveloped.