The Giant Panda Testicular Histology Structure And Semen Freezing

The giant panda, a protected species, is endemic to China and one of the rare animals in the world. The giant panda’s natural mating ability are very low, their number is very scarce, especially the lower rate of reproduction of captive giant pandas. Therefore, to improve frozen semen quality is the focus of many researchers. However, the use of artificial insemination still can’t improve the pregnancy rate of giant pandas significantly. After the study, it is found that the quality of the semen used in artificial insemination is the key to the female panda pregnancy. The artificial insemination is widely used in the breeding of giant pandas and has become an effective way to improve the rate of giant panda breeding. The reproductive system is the basis of animal reproduction and evolution. The study of its structure is the most basic work of animal ecology, reproduction and population biology. Sperm are the germ cells of male animals carrying the genetic material, they are able to fertilize an egg into a fertilized egg, and the fertilized egg can develop into an embryo. In the process of cryopreservation, the sperm are vulnerable to damage, affecting the sperm of normal metabolism and normal physiological functions and the ability of fertilization, resulting in low conception rate.To understand its composition and structural characteristics by observing the histological structure of testis pair of giant pandas. The observation of the structure of the mature sperm of the giant panda by using scanning electron microscope and scanning transmission electron Microscopy. The experimental results and conclusions are as follows:1. The reproductive system of male giant panda consists of testis, epididymis, spermaduct, penis and scrotum. Testis is located in the scrotum, oval and with smooth surface. Testes oval, which has a smooth surface, is located to the left and right sides of the lower part of the scrotum. The caput pale bowl is covered in the front of the testis, the surface of the epididymis takes the shape of flat ribbon is covering the testis measured close to the dorsal’s edge. Epididymal in the testes back-end is wider than the epididymal head down cover back-end surface in the testis. Epididymis is composed of the epididymal head, epididymal body and epididymal. The vas deferens is a small tube; it is a direct continuation of the epididymal tail. 2. In addition, the vas deferens, the organization of the giant panda is a slender tube, play a role in the transport of sperm. Membrane is in the inner layer, the sheath mold is in the outer sheath, and the albuginea is thicker than sheath mold seminiferous tubule is the main component composed of testicular tissues, but also produce sperm place. Its wall is composed of spermatogonia, primary spermatocytes, secondary spermatocytes, sperm cells and sperm.Under normal circumstances, the sperm will be gathered in the seminiferous tubule lumen central. The giant panda of the epididymis efferent ducts and epididymis tubes, the output has sperm in the lumen of tubules and epididymal duct. Testicular tissues of giant pandas outside clad with two layers of film-the tunica vaginalis and tunica albuginea, and together form the testicular tissue of the capsule.3. The giant panda sperm can be divided into three parts, namely the head, neck and tail. The giant panda sperm head is pear-shaped, his mouth nuclear matter, more pointed front end, and a cap-like structure covering the sperm head of the first half of this cap-like structure is the acrosome. The acrosomal is closely related to the structure and fertilization. While the sperm is contacting with the egg, the acrosome release contains lysosomes, and then lysosomes with ablation of the egg transparent sperm-egg binding to the form the fertilized egg. The neck of the giant panda sperm is very short, thin and long tail, the tail is responsible for sperm motility, because a large number of mitochondria existed in the tail of the sperm to provide energy for sperm motility in the sperm tail.4. According to the results of this experiment, trehalose and sucrose can play a role in the protection of giant panda semen cryopreservation process, but the protective effect is not the same. In contrast, trehalose is better than the cryoprotective effect of sucrose on the giant panda sperm. The thawed semen has higher sperm motility, sperm motility, sperm plasma membrane integrity rate and the integrity of the acrosome. According to the findings of this experiment, the trehalose and sucrose added to the giant panda semen frozen liquid, the optimal concentration of0.5mg/L and75mmol/L...