Effects Of Mannitol On Sperm Quality During Liquid And Cryopreservation Of Pig Semen

Cryopreservation of semen can prolong sperm preservation time,but artificial insemination with cryopreserved sperm leads to low birth rate and decreased number of offspring,mainly due to low temperature damage caused by hypothermia.Pig semen preservation methods are divided into liquid(17 ?)save cryopreservation(196 ?),save 2 kinds.Liquid form for short-term preservation,frozen form for long-term preservation.Pig sperm to cold stimulation,especially from sharp cooled to 10,0?,the temperature can make sperm happen not reversible cold shock phenomenon.Therefore,the cryopreservation agent must be added in the diluent to improve the sperm's ability to resist cold.The main purpose of this study was to detect cryoprotectants mannitol to the pig semen cryopreservation after liquid and sperm plasma membrane integrity,acrosome membrane integrity,sperm apoptosis gene expression and fine-eggs within the effects of combining ability.The test results are as follows:1,in the process of liquid preservation,adding mannitol in diluent(0,0.3,0.6 and 0.6 mg/ml)save after 5 d,live sperm rate,rate of plasma membrane integrity and acrosome membrane integrity rate have different degrees of weakening,but add 0.3 and 0.6 mg/ml of the treatment group compared with the Control group significantly increased(P<0.05).Sperm apoptosis gene Caspase 3,Caspase-8 and the expression of TNF alpha and semen quality test results the same,all have different degrees of improvement(P<0.05),therefore,mannitol is added in the normal temperature preservation process can obviously improve saved semen quality,improve the fertilization rate.2,in the process of cryopreservation,the temperature fell sharply,to damage the quality of sperm is opposite bigger,added in the cryoprotectants 1 mg/ml,2 mg/ml mannitol in the treatment group(39.31%,43.89%)semen frozen thawed,live sperm rate compared with the control group(39.89%)improved significantly(P<0.05),at the same time,1 mg/ml of dealing with the group of sperm plasma membrane integrity rate was 43.10%,2 mg/ml membrane integrity was 46.08%,the treatment group paper were significantly higher than that of control group(42.23%).There were significant differences(P<0.05).The results of sperm acrosomal integrity test were the same as that of plasma membrane integrity test,and the quality of sperm in the treatment group was higher than that of the control group.In addition,within the thawed sperm apoptosis gene expression quantity for testing,the experimental results show that adding 2 mg/ml least amount of sperm apoptosis gene expression in treatment group,explain the degree of sperm apoptosis is compared other groups,to sum up,in the process of adding mannitol in the frozen will protect sperm from combat damage,improve the quality of sperm.3.Mannitol has a protective effect on sperm preservation.The optimal concentration during normal temperature preservation is 0.6mg/ml,and the optimal concentration during freezing preservation is 2mg/ml.