| In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase (PAF-AH) activity and glutamic-aspartic aminotransferase(GOT) activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:(l)Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9,59.6, 56.2, 54.7, 47.3, 38.2, 30.8, 23.8, 18.4, 16.8,14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.(2) The presence of PAF-AH and GOT were found in all Giant panda seminal samples, their average concentration were 56.76±60.86 mmol/min/mL and 194.89±77.40 IU/100mL. Correlation analysis revealed a highly negative correlation between PAF-AH concentration and sperm motility(R= — 0.73, P<0.001), it also had negative correlation with sperm moving state (R=—0.58, P<0.05); seminal GOT concentration just has highly negative correlation with sperm motility (R=— 0.66, P<0.01). The results indicated that the concentration of PAF-AH and GOT in giant panda seminal plasma can supply a characteristic reference to evaluate semen quality.In this article, we also studied the effects of pentoxifylline (PF), platelet activatingfactor (PAF) and prostaglandin F2a(PGF2a) on giant panda frozen-thawed sperm fertility in vitro. Frozen-thawed sperm was incubated with these active components which were added to Ham's F-10 medium with different dosage at 37°C bath. The results indicated that:(1)PF^ PAF and PGF2a all can affect giant panda frozen-thawed sperm fertility in vitro, but the actions on sperm varies not only in different components but also in their different dosage. All data confirmed that lmg/mL PF was most suitable for improving giant panda frozen-thawed sperm fertility in vitro. When incubated the sperm with lmg/mL PF at 37"C bath, the living time of sperm was 15.33±4.73h, the living index was 3.77±0.54h, the heterogenetic penetration-egg ratio was 51.44% after incubating for 4 hours, the heterogenetic penetration-egg ratio was still 7.49% after incubating for 6 hours. The results were significant difference between the treated and control group (P<0.01) , also better than other treated groups.(2) Treated giant panda frozen-thawed sperm with 50ng/mL PAF had better effects than lOOng/mL PAF, but the sperm function would be damaged when incubation time exceeded 2 hours. So , the time of treating sperm with PAF should not be so long.(3) 50ng/mL PGF2a had no significantly bad effect on giant panda, but when increased PGF2a treated concentration, sperm fertility in vitro would be reduced because of the damaged motility and declined acrosome reaction ratio.(4) In the hypoosmotic swelling test(HOST) of giant panda frozen-thawed sperm, the percentage of sperm tail swelling showed a significant positive correlation with sperm motility(r=0.78, P<0.05) ,also showed a highly significant positive correlation with sperm heterogenetic penetration-egg ratio (r=0.90, P<0.01) .The results indicated that HOST can be used as a method to examine giant panda sperm membrane functional integrity. |
PDF Full Text Request