| The Buyanghuanwu decoction was a classical prescription for the treatment of the deficiency of Qi and blood stasis in traditional Chinese Medicine, which had a significant effect on cerebral ischemia, stroke, thrombosis, blood fat and improving immunity. This composition is numerous, complex and its validity basis and mechanism of action has not been elucidated. If we could build an effective research method adapted with tradition Chinese medicine compound characteristic, it was expected to provide new ideas and evidence for the Chinese medicine compound modernization study.Purpose:Take the Buyanghuanwu decoction after water extraction and alcohol precipitation as model drugs, this paper was intended to count the spectrum efficiency relationship of more ingredients corresponding more targets on resistance to nerve cells injury and apoptosis of brain ischemia symptomatic, which was used by its Chinese traditional medicine composition fingerprint and its pharmacodynamics experiment, bonded with the spectrum efficiency learn mathematics model, which based on Hill concentration-response equation combined with mathematical statistical analysis principle. It was hoped to provide new thinking, method and experimental basis to TCM compound effectiveness research.Methods:the key point is to examine and evaluate the spectrum efficiency relationship of more ingredients corresponding more targets on resistance to nerve cells injury and apoptosis of brain ischemia symptomatic.1. It established the TCM compound spectrum efficiency mathematics model initially based on Hill concentration-response equation combined with mathematics basic principle, statistical analysis method and the computer C language program.2. The fingerprint of Buyanghuanwu decoction after water extraction and alcohol precipitation was detected by HPLC, and the concentration determination method of ligustrazine, hydrochloric acid, amygdaline, herbaceous peony glucoside and ferulic acid was investigated by it.3. The spectrum metabolism characteristics of Buyanghuanwu decoction after water extraction and alcohol precipitation was investigated in vivo in SD rat.4. The different polarity composition groups in Buyanghuanwu decoction were obtained by the solubility parameter method and their fingerprint characteristics and similarity were evaluated.5. Through the star design for the investigation and evaluation of Buyanghuanwu decoction after water extraction and alcohol precipitation resistance to cerebral ischemia nerve cells injury and apoptosis, the spectrum efficiency relationship parameters were calculated by the previous spectrum efficiency mathematical model.Results:1. RP-HPLC methodology for fingerprint characteristics of Buyanghuanwu decoction after water extraction and alcohol precipitation was established with appropriate convenience and accuracy.2. The metabolism apparent half-life of Buyanghuanwu decoction after water extraction and alcohol precipitation was9.108h in rat in vivo, and it metabolized95%though9.108±1.96x9.062h after infusion.3. The different polarity composition groups for Buyanghuanwu decoction could obtain by11solvents processing with different solubility parameter, and the fingerprint similarity between different processing samples was remarkable differently, which showed different composition category and content.4. Combined with spectrum efficiency mathematical model, HPLC fingerprint and pharmacodynamics experiment, it has obtained a series of spectrum efficiency parameters of Buyanghuanwu decoction after water extraction and alcohol precipitation resistance to nerve cells injury and apoptosis of brain ischemia symptomatic. The spectrum efficiency mathematical model built before has been checked. In this experiment, spectrum efficiency parameters for every ingredient were of big difference, which showed that their targets and strength of the function were dissimilar.Conclusion:This study had validated the established spectrum efficiency mathematical model preliminary of Chinese herbal compound composition, which could be used in the Chinese herbal composition effectiveness research with many compound and many targets characteristics, and provide new ideas and experimental basis to the modernization of traditional Chinese medicine. |
PDF Full Text Request