Abalone Mushroom Polysaccharide Structure And Its Pharmacological Activity

Research shows that edible fungus polysaccharide has the remarkable enhancement immunity, lowering blood pressure, lowering blood lipid, antioxidant and antitumor activity. Pleurotus abalonus Han, K.M.Chen et S.Cheng is a traditional Chinese medicinal and edible fungus distributed in Fujian and Taiwan Province of China, which is a Pleurotus genus fungus of Tricholomataceae belonging to the Basidiomycetes and some biological activities have been reported.At present, the study of P. abalones is still in the initial stage in home and aboard. In this article, we study the extraction, separation and purification, structure and biological activity of polysaccharide from P. abalones (PAP) purchased Fujian Province in-depth.1. Obtained the PAP by method of ultrasound-assisted hot water extraction. Response surface analysis methodology (RSM) was used to optimize the extraction process of polysaccharides from P. abalones, employing a four-level and four-variable Box-Behnken design. And by solving the regression equation and also by analyzing the response surface contour plots, the optimal process parameters were determined:power of ultrasonic was165.46W. time was57.89min, temperature was71.41℃, ratio of solution to material was25.78, and in order to practical convenience, we adjust the optimal conditions to power of ultrasonic was165W, time was60min. temperature was70℃. ratio of solution to material was26:1. Under the optimal conditions the corresponding response value predicted for polysaccharide production was3.42%. which was confirmed by validation experiments. Moreover, the total sugar content of was67.0%.2. In this study we removed protein form PAP by Sevage method, used activated carbon adsorption. And then polysaccharide from removing proteins and pigments through DEAE-52cellulose column chromatography obtained further purification to get three elution peaks. The more elution components of0.05M and0.1M NaCl were pured by G-150dextran gel column chromatography and washed for homogeneous elution peak. Column chromatography got two homogeneous elution peaks, dialysing by freeze-dried. There were two refined polysaccharides named in PAP-1and PAP-2. Then we analyse the physical and chemical properties and the structure of PAP-1and PAP-2preliminarily. Physical and chemical properties test and UV wavelength scanning results showed that the two components do not contain starch, protein, amino acids, peptides and nucleic acids and other impurity. Infrared pop scan displayed that the two components are heteropolysaccharides. PMP pre-column derivatization HPLC-UV analysis results indicated that the monosaccharide composed of PAP-1are D-mannose. L-rhamnose, D-glucose and D-galactose with the molar ratio of1.08:1.00:84.58:2.09, and the monosaccharide composed of PAP-2are D-mannose, D-ribose; L-rhamnose. D-glucose, D-galactose with the molar ratio of3.18:1.00:1.76:81.58:4.09. High performance liquid chromatography gel permeation chromatography analysis showed that the molecular weight of PAP-1, PAP-2were18W Da,15W Da, respectively.3. We evaluated antioxidant activity of PAP-1. PAP-2by the scavenging ability of DPPH*free radical, ABTS free radical, superoxide ion free radical, hydroxyl radical free radical, reducing power and ferrous ion chelating activity in vitro. The results showed that PAP-1, PAP-2have a certain antioxidant activity with a good dose-effect relationship. In conclusion, PAP-1, PAP-2displayed strong scavenging activity on hydroxyl free radical free radical and superoxide anion free radical.4. We evaluated antitumor activity of PAP-1, PAP-2by in-colon cancer cell line LoVo in vitro. The results indicated that PAP-1, PAP-2could inhibite LoVo cell’s growth, induce LoVo cells accelerate the release of lactate dehydrogenase LDH, also induce apoptosis in LoVo cells and LoVo cell growth arrest in S phase, and they are effected by LoVo cells and reactive oxygen species (ROS) level, and showed a dose-effect relationship. But the mechanism of induced LoVo cell apoptosis and cycle block is not clear, and further study is necessary to confirm it.