Structure Of Polysaccharides From Pleurotus Ostreatus And Its Analysis Of Anti-Aging On Mice Induced By D-galactose

Pleurotus ostreatus,one of the most widely cultivated edible fungi,produces about 60%of its annual crop.Aging is accompanied by many diseases,and there is no effective treatment,so it is very important to find effective natural anti-aging substances.In this study,Polysaccharides of Pleurotus ostreatus(PP)were isolated and purified by water extraction alcohol precipitation,deproteinization,chromatography and dialysis.In mouse model induced by D-galactose,the anti-oxidation,anti-inflammation and anti-apoptosis of brain and liver were analyzed and pathological sections of brain,liver and colon were observed.The structure of polysaccharides(PP)was analyzed by Gas chromatogram ? Fourier-transform infrared and Nuclear magnetic resonance.The main results are as follows:(1)Antioxidant analysis of PP in vivo: Superoxide dlsmutase(SOD)activity and Malondialdehyde(MDA)content were used as antioxidant indexes in brain and liver tissues.The results showed that after PP intervention,SOD activity in brain and liver tissues respectively increased by 25.8 U/mg prot and 36.23 U/mg prot and MDA content decreased by 1.78 nmol/mg prot and 2.71nmol/mg prot respectively,indicating that PP can improve the antioxidant capacity of D-galactose-induced aging mice some extent.(2)Anti-inflammatory analysis of PP in vivo: Using interleukin-1?(IL-1?),interleukin-6(IL-6),and Tumor necrosis factor-?(TNF-?)as inflammatory indicators,the expression of mRNA in brain and liver tissues by the real-time fluorescence quantitative showed that PP could reduce the expression of IL-1?,IL-6 and TNF-? mRNA in D-galactose-induced aging mice.The results suggested that PP can enhance the anti-inflammatory effect in D-galactose-induced aging mice.(3)Anti-apoptotic analysis of PP: Using antibody of apoptotic proteins Bax,Caspase-3 and anti-apoptotic protein Bcl-2,Western and immunofluorescence sections were performed to observe brain tissue,and immunofluorescence sections were performed to observe liver tissue.It was found that PP could reduce the expression of Bax and Caspase-3 in brain tissue and increase the expression of Bcl-2;the expression of Bax in liver tissue was decreased and the expression of Bcl-2 was increased,which proved that PP could enhance the anti-apoptoticability of aging mice.(4)Pathological section analysis: The observation of H&E tissue pathology slices showed that brain ?liver and colon of the aging mouse induced by D-galactose appeared different degree of damage,including decrease of hippocampal tissue contour cell,cell degeneration?cytoplasm osteoporosis and cell edema of liver tissue,intestinal lamina propria significantly shorter and mucosal muscularis dispersion of colon tissue.After the intervention of PP,the above symptoms were eased,showing that PP has certain repair function for the injury of brain,liver and colon of aging mice induced by D-galactose.(5)structure analysis of PP: The result of Gas chromatogram(GC)showed that PP is composed of mannose,ribose,glccuronic acid,glucose,galactose,xylose and fucose with mass ratios of 15.78%,0.91%,1.10%,57.62%,23.77%,0.14% and 0.67%.Infrared spectroscopy(FT-IR)showed that PP was the glycoside bonds of ? and ?.The result of Nuclear magnetic resonance(NMR)detected that 5.44,5.41,4.94 and 4.87 ppm anomeric of PP may represent four kinds of main monosaccharides,such as mannose,glucose,galactose and xylose,and carbon spectrum 103.70,102.64,99.31 and 93.55 ppm in four anomeric carbon,respectively may be ?-galactose,?-mannose,?-mannose and ?-glucose.In summary,PP has strong antioxidant,anti-inflammatory and anti-apoptotic activities,and has a certain repair effect on tissue damage induced by D-galactose in aging mice,which provides a reference for the development of natural anti-aging drugs for the body.