| Objective:To research the effect of abdominal vibration therapy on glucose metabolism and the GLP-1of T2DM rats. Using the results obtained, discuss the mechanism of abdominal vibration therapy’s effect on T2DM from the angle of "abdominal technique-intestines-pancreatic island axis" and therefore provide experimental evidence for the clinical application of abdominal vibration therapy preventing or treating T2DM.Method:After1week of acclimatization,30healthy8week old male Wistar rats (250g±10g) were randomly divided in two:a control group and a model group. Rats in the control group were fed normal laboratory rat chow while rats in the model group were fed with chow containing high amount of sugar and fat.4weeks later, the model group received a single i.p. injection of STZ (30mg/kg). while the control group received a single i.p. injection of citric acid-sodium citrate buffer solution of the same volume. Rats were raised for another2weeks and non-fasting blood glucose levels were tested then an OGTT was performed. Rats which met the T2DM standard were randomly divided into the model group and the abdominal vibration group. Abdominal vibration therapy was performed once daily for10days, then2days of rest followed by a second treatment period of10days. During treatment animals were bound to the table, the model and control groups were also bound for the same period of time but did not receive treatment. Blood glucose, cholesterol, triglyceride, fasting insulin, glucose tolerance and GLP-1were measured. Using these results the insulin sensitivity index was calculated and glucose metabolism and GLP-1was compared between the groups. Pancreas of all rats were fixed with formaldehyde then cut into sections and dyed to observe pancreatic islet cell histology and take pictures for comparison between groups.Results1. Effects of abdominal vibration therapy on behavior and weight of T2DM ratsDiabetic rats drank and urinated more. Litter became dirty easily needing frequent changing. The T2DM rats gained weight much faster, however total weight gain was not significantly different between the groups (P>0.05). After the2week treatment period, rats in the model group lost weight and T2DM symptoms became more serious, meanwhile the abdominal vibration group’s mean weight remained the same.2. Effects of abdominal vibration therapy on the glucose metabolism of T2DM rats After the treatment, compared with the model group, the ISI and OGTT of the abdominal vibration group rose significantly (P<0.05). FBG dropped significantly (P<0.05) and there were dropping trends in FINS. Compared with control group, FBG showed no significant difference (P<0.05).3. Effects of abdominal vibration therapy on GLP-1of T2DM ratsAfter the treatment, GLP-10-30min after glucose gavage in the model group and abdominal vibration group dropped dramatically (P<0.05) compared to the control group. There was no significant difference between the amounts of serum GLP-1among three groups at0min and30min (P<0.05).4. Effects of abdominal vibration therapy on insulin and pancreatic islet cells of T2DM ratsAt the beginning of treatment, FINS had no significant difference between groups (P<0.05). however the FINS average in the model and abdominal vibration group was higher than the control group.After the treatment, there was no significant difference of FINS among three groups (P<0.05). Compared with the control group, the average FINS in the model and abdominal vibration group showed a dropping trend and the abdominal vibration group dropped more seriously than control group. A conclusion can be drawn that pancreatic β-cells of the model group were damaged lightly but long lastingly, while abdominal vibration alleviated this damage.It can be observed that pancreatic islet cell damage in the abdominal vibration group is lighter than model group. Cells from the abdominal vibration group were regular in shape, even, complete in form and number, and nucleus and boundaries were clear. Fat cells were fewer in the abdominal vibration group.Conclusions1. Abdominal vibration therapy can alleviate high blood glucose and T2DM symptoms.2. Abdominal vibration therapy could alleviate high blood glucose through protecting pancreatic islets and increasing insulin sensitivity.3. The results of this research could not prove that abdominal vibration therapy improves blood glucose via influencing GLP-1, further research is needed.4. T2MD rat model induced by high amounts of sugar and fat chow combined with single injection STZ (30mg/kg), was80%successful. |
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