Thistle Stem Leaf Chemical Components And Content Of Research

The plant Tribulus terrestris L., belonging to the family Zygophylianceae, have been used in traditional Chinese medicine against various diseases. The stems and leaves of Tribulus terrestris L., also known as "Jili-miao", only contained in the "Shen Nong’s Herbal Classic" are used for treating edema, abdominal distension and so on. As we all know, besides the magical therapeutic effect of the fruit of Tribulus terrestris L., its roots, flowers, stems and leaves can also be used as medicine parts. Tribulus terrestris L. has contained numerous compounds such as saponins, flavonoids, alkaioids and polysaccharides. Modern pharmacological studies have shown that steroidal saponins and flavonoids are mainly active constituents, which can improve cardiovascular diseases, lowering blood sugar, enhancing sexual function, anti-aging and lipids, anti-neoplasm pharmaceutical activity.Firstly, the paper summarizes the research situation of chemical constituents and p harmacological activities on the basis of the current domestic and international literatur es (153references were cited). By means of macroporous resin, MCI gel, the Sephade x LH-20, preparative HPLC separation method et al.14compounds were isolated from the95%EtOH extract of stems and leaves. Among them, on the basis of spectroscop y analysis, including UV,1HNMR,13CNMR, HMBC, HSQC and chemical methods,5compound structures were identified as isorhamnetin-3-O-β-D-gentiobioside (JL-1), querc etin-3-O-β-D-gentiobioside (JL-2), kaempferol-3-O-β-D-gentiobioside (JL-3), isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-4), kaempferol-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-5), N-trans-feruloyl tyramine-4’-O-β-D-glucopyranoside(JL-6). Among the m, compound JL-6was the first reported in Zygophylaceae. Compounds JL-1, JL-2an d JL-4with the purity of more than99%were chosen as the reference substance. Esta blish a quantitative method for the determination of three flavonols in the effective of the stems and leaves of Tribulus terrestris by HPLC-ADA. The contents of index com pounds including isorhamnetin-3-O-β-D-gentiobioside (JL-1), quercetin-3-O-β-D-gentiobio side (JL-2) and isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-4) were deter mined by HPLC. The calibration curve is linear at ranges of0.56-14.00,3.24～129.60and0.92～23.00μg/mL respectively. Their regression equations are Y=21388X-2087.8, r=0.9996; Y=34596X-37335, r=0.9998; Y=50667X+10439, r=0.9998。In this paper, by the isolation and structural identification of flavonoids compound from the stems and leaves of Tribulus terrestris L, we confirmed higher content of th ree flavonol compounds as reference substance. Establish a method based on reversed phase HPLC was developed to simultaneously determine the content of isorhamnetin-3- O-β-D-gentiobioside, quercetin-3-O-β-D-gentiobioside and isorhamnetin-3-O-β-D-gentiobio side-7-O-P-β-glucoside. This method is considered to be simple, accurate, stable, good precision and reproducibility and is suitable for evaluating the quality of Tribulus terre stris L.