Motility Insomnia Rat Model

1Objective:Through the comparison of different modeling methods, establish a relatively complete the movement of the insomnia rats model for the study of the mechanism and cure exercise-induced insomnia lay the foundation of the important.2Method:This experiment selection female Wistar rats health only85, will rats randomly divided into seven groups:normal group,3%movement PCPA group,5%load injection sports load PCPA injection group,7%load group,7%movement sports load PCPA injection group, Y maze training group, Y maze training injection PCPA groups, and only10. Besides the normal group will not do anything besides,3%movement PCPA group,5%load injection sports load PCPA injection group,7%PCPA groups to exercise load injection5weeks3%,5%and7%weight loading swimming training+intraperitoneal injection of PCPA;7%of sports load5weeks7%weight loading swimming training; Y maze PCPA groups to training injection5weeks Y maze training+intraperitoneal injection of PCPA; Y maze training group to5weeks Y maze training. The rat pick eye take blood, add heparin for the administration. Through the high performance liquid chromatography (HPLC) method of plasma fluorescence determination of monoamine transmitters in content. All the data SPSS17.0statistics software statistics processing.3Result:3.1the change of the body surface characteristics of ratsY fan group,7%in the days and nights v of the phenomenon, but to the outside world, lip color induction ability, shape, fur and grabbed a strong resistance level performance and7%+PCPA rats, great difference in the body surface characteristics;5%+PCPA rats, although frequent activity, a series of surface characteristics are7%and+PCPA rats have obvious difference; Y+PCPA group and3%+PCPA rats, activity rules performance is not obvious, and a series of surface characteristics are7%and+PCPA rats, surface characteristics are quite different.3.2each rat weight change(1) the rats within the group week3and4weeks compared weight,7%group difference is very significant (P<0.01) weight fell sharply,5%+PCPA group significant difference (P<0.05), weight to drop; The rats in the group4weeks and5weeks compared weight,7%+PCPA group significant difference (P<0.05), weight to drop.(2) the fifth week each rat weight and7%+PCPA group, compared to the normal group difference is very significant (P<0.01), normal group and a growing trend, other each significant difference (P<0.05), to drop.3.3each rat food intake of change The rat week3and4weeks food intake change compared:7%+PCPA rats change very significant (P<0.01), is a sharp decline,5%+PCPA rats, change significantly (P<0.05), the decline of other group no change;The rat4weeks and fifth week food intake change compared:7%+PCPA, Y fan rats, change significantly (P<0.05), the decline of5%+PCPA, Y+PCPA rats, change very significant (P<0.01), is a sharp decline.5weeks each rat food intake and7%+PCPA compared:normal rats, change very significant (P <0.01), is a sharp decline of5%,7%,3%++PCPA group, PCPA group and Y+PCPA rats, change significantly (P<0.05), to drop.3.4each rat plasma NE, DA content changes(1) rats plasma NE content, compared with the normal group,7%+PCPA group, the7%rise very significant group,(P<0.001),3%+PCPA group (P<0.05); And7%+PCPA group, compared to7%,5%+PCPA group group,3%+PCPA group, Y fan group and Y+PCPA group significantly lower than7%+content PCPA group (P<0.001); Significantly lower than3%+7%group PCPA group (P<0.05) and very significantly lower than5%+PCPA group, Y fan group (P<0.01);5%+PCPA group very significantly higher than Y+PCPA group (P<0.01); Y fan group significantly higher than Y+PCPA group (P<0.05).(2) rats plasma DA content, compared with the normal group,7%+PCPA group significant higher than normal group (P<0.001),7%very significantly higher than normal group group (P <0.01); And7%+PCPA group, compared to5%+PCPA group,3%+PCPA group, Y fan group, Y+PCPA group very significantly lower than7%+PCPA group (P<0.01),7%were lower than7%+PCPA group group (P<0.05); Compared with7%group,5%+PCPA group, Y fan group, Y+PCPA significantly less than7%of group (P<0.001),3%+PCPA group significantly lower than7%group (P<0.05).4Conclusion:This experiment with large load swimming training (sports time:90minutes, exercise load:7%of their body weight)+PCPA intraperitoneal injection of rats caused insomnia models, from external surface characteristics, rats weight, food intake and plasma monoamine neural transmitter NE and DA changes of the contents of the feature, accord with Chinese medicine virtual fatigue symptoms of insomnia, that the use of this method to establish insomnia rat model feasibility strong, can be widely used in sports and clinical aspects of insomnia insomnia basic research.