Studies On The Synthesis,Characterization And Application In Immune Of Thoil Schiff And Complexes

In this thesis, the Schiff base has good antibacterial properties and potential pharmaceutical value, designed and synthesized on the basis of previous work phthalaldehyde and β-mercaptoethylamine two series containing sulfhydryl Schiff base transition metal complexes, and to characterize the structure of found synthesis mercapto Schiff base and its transition metal complexes are the target product, and has good biological activity and medicinal value.Prepared by self-assembly method shrink3,5-the dibromo alkali nickel complexes with high biological activity unmarked impedance type h-IgG immune sensor; sodium citrate reduction method based on beta-mercaptoethylamine gold nanoparticles unmarked biological immune probe optimization and selection of h-IgG quantitative detection analysis curve.10ng/mL～100ng/mL h-IgG concentration range, linear equations ARP=-60.23+1.147×1011×Ch-IgG, R2=0.9997,4.54×10-10g/mL detection workPrepared by self-assembly method based on optimized phthalaldehyde shrink o aminothiophenol Schiff base nickel complexes with high biological activity unmarked impedance CRP biological immune sensor; unmarked biological immune probe select, determine the working curve of the analysis of quantitative detection of Ab-CRP.20ng/mL～1000ng/mL Ab-CRP concentration range of linear equations for ARp=-1301.6+6.75×1011×CAb-CRP, R2=0.9992,3.56×10-9g/mL detection work limit.phthalaldehyde the aminothiophenol mercapto Schiff base fluorescent molecular switch of ciprofloxacin hydrochloride detection system found in the4×10-4mol·L-～1×10-2mol·L-1concentration range, developed Schiff alkali fluorescent molecular switch work curve linear fitting linear equation AF=111.17+2.229×104×CCPFX, the linear correlation coefficient R=0.9997, the lowest detection limit to3.26×10-5mol·L-1.Six kinds containing thiol Schiff base ligands as means to study the fluorescence emission spectra, using the method of density functional theory (DFT) mercapto Schiff base ligand geometry of the full range of parameters through quantum chemical calculations optimized by using time-density functional theory (TD-DFT) methods to further optimize energy mercapto Schiff Base Molecular, which later generations to study such thiol Schiff base fluorescence emission spectra provide theoretical guidance.