Study On Lignans-extraction Techniques By Microbial Fermentation Of Flaxseed Cake

As a kind of natural phytoestrogens, lignans play an important role in anticancer andanticardiovascular disease. Therefore, it is considered to be useful for anti-cancer, anti-tumor,anti-cardiovascular diseases. Flaxseed is a rich source of lignans, however, cyanogenic glycosides inflaxseed can release extremely poisonous hydrocyanic acid because of the catalytic decompositioneffect of intestinal β-glucosidases. So, we used flaxseed cakes as raw material, and the research inextraction and separation methods was carried on with the aim of high yield and lower cost. After studyon microbial meal, it is found that the microbial fermentation method has a role in degradation ofcyanogenic glycosides, and detoxification technique of flaxseed cake is in research. The achievementswere as follows:(1)The effects of fermentation time, inoculation amount, and fluid-solid, on yield ofsecoisolariciresinol diglucoside(SDG) from flaxseed cake by microbial fermentation were studied andthe parameters for optimum yield were obtained by using RAS software. The results showed that theoptimum parameters were: fermentation time was5.55days, inoculation amount was1.28%, andfluid-solid was51.28%. The SDG extraction rate was51.89mg/g under these conditions.(2)The microbial fermentation methods were employed to detoxify flaxseeds. The L49(3)orthogonal array was adopted to the experiment. The results showed that the optimum parameters were:liquor-making yeasts, fermentation time was72h, inoculation amount was3%, fluid-solid was50%, andfermentation temperature was28℃. The degradability of cyanogenic glycosides was76.91%underthese conditions.(3) The UPLC (Ultra Performance Liquid Chromatography)for the extraction of flax lignans fromflaxseed was established: ultravlolet detector(deteetionwavelength:283nm); Symmetry C18aschromatographic column(1.7um，100×2.1mm); column temperature: room temperature; mobile phase: Ais0.1%phosphoric acid, B is acetonitrile; veloeity:0.208mL/min; sample size:2.5ul. thoughverification tests, show that the method has good stability and reproducibility, and thesecoisolariciresinol diglucoside(SDG) has a good resolution.