Study On Simultaneous Determination Of8Kinds Of38Veterinary Drug Residues In Aquatic Products

Veterinary drug residue, refers to edible animals after using of veterinary drugs,accumulated or stored in the animal cells, tissues or organs, such as the prototype drug,toxic metabolite or impurities. Veterinary drugs have a good effect on prevention andtreatment of animal diseases, improving the efficiency of breeding, etc. Quinolones,macrolides, sulfonamides, trimethoprim, lincosamides, nitroimidazoles, quinoxalinesand peptides compounds are currently used in animal husbandry and aquaculture,However, abuse of these drugs could easily lead to serious pollution of aquaticproducts and edible animals, they can accumulate in the human body through the foodchain, and have cause allergic reactions and make huaman body become resistanceand other side effects. Therefore, monitoring veterinary drug residues in the animalproducts, especially the safety of aquatic products has a positive meaning.In this paper, the eels, bighead carp, crayfish, catfish fry langouste and otheraquatic products are the analytical objects, we have researched the simultaneousdetermination of quinolones, macrolides, sulfonamides, trimethoprim, lincosamides,nitroimidazoles, quinoxalines and peptides residues. The article consists of threechapters, the details as follows:In Chapter One: Introduced the physical and chemical properties and the harm ofquinolones, macrolides, sulfonamides, trimethoprim, lincosamides, nitroimidazoles,quinoxalines and peptides residues, summarized the study of veterinary drug residuescommonly used analysis methods at home and abroad and its dynamics. Thedetermination of the veterinary drug residues usually contains two processes: samplepretreatment and detection. It contains extraction, purification, concentration,enrichment. For purification methods, this article introduces liquid-liquid extraction(LLE) and solid phase extraction (SPE) methods. The methods for the determinationof the residues have gas chromatography (GC) and gas chromatography-tandem massspectrometry (GC-MS), thin-layer chromatography (TLC) and high performance thinlayer chromatography (HPTLC), supercritical fluid chromatography (SFC), capillaryelectrophoretic(CE), high performance liquidphase chromatography (HPLC),chromatography-mass spectrometry.In Chapter Two: A new approach was established for the simultaneousdetermination of eight classes limited using veterinary drug residues, includingquinolones, macrolides, sulfonamides, trimethoprim, lincosamides, nitroimidazoles,quinoxalines and peptides, in aquatic products by high performance liquidchromatography-tandem mass spectrometry (HPLC-MS/MS). The analytes wereextracted with1%acetic acid in acetonitrile, then purified with refrigerated centrifugeand hexane. Sample matrix-matched calibration was used to determine the residue contents by the external standard. Electrospray ionization mass spectrometry wasoperated in the positive mode using multiple reaction monitoring (MRM) for thequalitative and quantitative analysis of38analytes. The result indicated thatcorrelation coefficients of the calibration curves were more than0.99. The averagerecoveries of38analytes at four spiked concentration levels ranged from42.2%to122.5%, The intra-day relative standard deviations (RSDs) were in the range of1.0%-26.4%, and the inter-day RSDs were1.6%-28.9%. The limits of quantitation(LOQs, S/N≥10) were in the range of5-20μg/kg. The method is simple, rapid,reliable and is suitable for the simultaneously determination of multiresidues ofveterinary drugs in aquatic products.In Chapter Three: summarized the main methods of this study, and looks forwardto the follow-up development insufficiency of experiment.