| This experiment use the walnut shell as raw material, the extraction process of walnut quinone and its content was determined:ingredients in the extraction were analysed,and the antioxidant activity of crude extract was determined in vivo and in vitro.The results are as follows:1. The extraction technology of walnut quinine was selected, by response surface analysised method the optimizal parameters of walnut quinine extraction is:the material was separated in24times ethanol and treated with250W ultrasonic at45℃for1.4h.The average yield of walnut quinine in this condition is8.1503%.2. Ingredients analysis of walnut shell:Concluded that walnut shell extracts of ethanol and chloroform phase contain low amounts of walnut quinone, and walnut shell walnut quinone content in the chloroform extract was slightly higher than ethanol。The result of GC-MS show:The main component in walnut shell extracts of ethanol and chloroform phase are oleic Acid (oleic Acid), stearyl Acid, hexadecanoic Acid),5-hydroxy-1,4-naphthoquinone (5-hydroxy-1,4-naphthaoquinon).3. The antioxidant activity in vitro was determined free radical scavenging ability and reducing power.The result shows:the effection show strong scavenging ability for DPPH·and02-·free radical.the scavenging ability is84.91%and80.36%, respective at2mg/mL ethanol parts,93.42%and90.64%for chloroform parts.Reducing power is1.971and0.998for each extract parts.All the two extract parts show very low scavenging ability for OH and H2O2free radical.4.Walnut shell extract antioxidant in vivo experiment:there is no significant difference (p>0.05) in the blank group and the control group of each index,means trace ethanol is harmless for mice. Alcohol extract has obvious removal of mice liver, kidney, heart and serum.the MDA content were decresed significantly (p<0.05or p<0.01) after35days treatment by ethanol parts.The activity of T-AOC, SOD, GSH-Px and CAT were increase (p<0.05or p<0.01).And show dose response rations in all targets. |
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