Stuty On Immunomodulation Efficacy Of Stachyose-lactic Acid Bacteria Synbiotics And Proliferation Mechanism

Synbiotics is a kind of microbial agents composed of prebiotics and probiotics, boththe type, quantity and their physiological effects of probiotics and prebiotics are importantfactors affecting immunomodulatory function.Therefore, it is necessary to study theimmunomodulation efficacy of stachyose-lactic acid bacteria and proliferationmechanism,which has important theoretical significance for development of newsynbiotics.In this study, nineteen dominant lactic acid bacteria isolated from diferent milk whichwere made by nomadic families in XinJiang were identified by conventional identificationand16S rRNA sequencing analysis. Stachyose concentration, types of carbon sources andaddition time of stachyose as single factors, the optimum proliferation condition ofLactobacillus plantarum RB₁obtained through the orthogonal tests. Then, as the object of studyin immunosuppressed and immunocompetent mice, studied the immunomodulation efficacy ofstachyose-lactic acid bacteria to the two kinds of mice. Finally, the proliferationmechanism of stachyose to RB₁was explored by measuring the amount of residual sugarin the fermentation broth, lactic acid content, activity of energy metabolism key enzymeand cell. The main results were as follows:1. Isolation and identification of the dominant lactic acid bacteria from Xinjiangyogurt. The test results showed that there were ten strains of Lactobacillus fermentum, fourstrains of Lactobacillus plantarum, four strains of Lactobacillus casei, one strains ofLactobacillus pentosus.2. The optimization of proliferation conditions of lactic acid bacteria fermentingstachyose. Lactobacillus plantarum RB₁was chosen to be probiotic which was component of Synbiotics. The optimum fermentation condition obtained through the orthogonal testswas using lactose as carbon and adding0.6%stachyose after inoculating4h. In thiscondition, the viable counts of RB₁in fermentation liquid was1.86010¹⁰cfu/ml whichwas as nearly8.49times as2.1910⁹cfu/ml before optimized.3. Effects of the immunomodulation efficacy of Stachyose-Lactobacillus PlantarumSynbiotics on immune function in immunosuppressed mice. The results show thatsynbiotics could promote the phagocytotic functions of mononuclear macrophage, DTH,the ability of antibody production, the index of immune organs, and it could also increasethe levels of serum IL-10, IL-12, IgG and TNF-α except for IL-12. Therefore,Stachyose-Lactobacillus Plantarum Synbiotics can remarkably improve immune functionin immunosuppressed mice such as cellular immunity, humoral immunity and non-specificimmunity, and raise index of immune organs and the level of some immune factors inserum.4. Effects of the immunomodulation efficacy of stachyose-Lactobacillus PlantarumSynbiotics on immune function in immunocompetent mice. The results show thatsynbiotics could promote the DTH, the ability of antibody production, the index ofimmune organs and increase the levels of serum IL-10, IL-2, TNF-α, IgMand TNF-α,andthere was no significant effect on the monocyte macrophage phagocytosis ofimmunocompetent immunocompetent. Therefore,Stachyose-Lactobacillus PlantarumSynbiotics can remarkably improve immune function in immunosuppressed mice such ascellular immunity, humoral immunity and raise index of immune organs and the level ofsome immune factors in serum.5. The proliferation mechanism of RB₁for fermenting stachyose. The results showthat stachyose raised the corbon source utilization rate of Lactobacillus Plantarum RB₁,generation amount of lactic acid, activity of α-galactosidase, lactate dehydrogenase,pyruvate dehydrogenase and cell metabolism. The resulting, the increasing of activity ofkey energy metabolism enzymes and cell metabolism may be one of the reasons for theproliferation of RB₁for fermenting stachyose.