Screening Of Fermentation Bacteria Of Pig Farming Waste And Performance Evaluation Of Fermentation

As China’s intensive animal farming to changes in trends, large amounts and concentratedlivestock waste disposal has become an important topic in recent years. Rise of fermentation bedtechnique brings new ideas that is a holistic solution. Using microbial technology, translatelivestock waste into fermentation bed that the live feed for pig, which can achieve the livestock andpoultry wastes fermentation, zero-emission standards, also to a certain extent, which can save thecost of farming and has a good environmental and economic benefits.First,we conducted an analysis of bacterial community structures of pig-fermentation-bed inthis topic, gene libraries of three levels of fermentation mattress were set up,The results showedthat there is a obvious bacteria diversity in the surface of the fermentation bed. Mostly, unculturedmicroorganism appeared, which accounted for39.5%of the total45strains detected. The othersbelong to13different species of bacteria and no predominant bacteria appeared. In the middlelayer of fermentation bed sample, uncultured microorganism accounted for15.5%of the total45strains detected. The others consisted of10different species of bacteria. The predominant bacteriaare Rhodanobacter sp. and Frateuria sp. respectively. This composition is associated with highcontent of ammonia nitrogen in fermentation substrate and oxygen content of the fermentation bed.The uncultured microorganism was not detected in all44bacteria strains in the bottom offermentation bed. There are14different bacteria genus in this layer. The predominant bacteria isRhodanobacter sp., which accounted for47.7%in all the bacteria detected. The others are in a lessproportion of total microbial population in this layerSecondly, through appropriate screening method for design, we have isolated photosyntheticbacterium, Bacillus, and Actinomycetes from the surrounding environment, and conductedresearch on the characteristics of three strains of bacteria. The part test results indicates that threestrains of photosynthetic bacteria have a degrading effect of N, P, S, strains Psb-3has a short lagphase,respectively the highest metabolic rates of N、P and S can be achieved96.38%、and15.8%and46.58%;Bacillus BX1and BX2has a shorter growth cycle, easy to cultivate, and capable ofproducing amylase and protease, In fermentation culture medium,Bacillus BX2producing amylaseand protease can reach78.70U/g,19.76U/g respectively; We have isolated three strains ofActinomycetes with uses fiber as the sole carbon source, and three strains of Actinomycetes againstfungi and bacteria have different degrees of inhibition. CLA-3inhibition of Escherichia coli,Staphylococcus aureus strains which works best, and in simulated fermentation, strain of cellulaseproduction capacity up to25.70U/g. Selection of superior strains--Psb-3, BX2, and CLA-3, withseparate molecular identification, results showed that strains Psb-3was Rhodobacter Sulfidophilus, strain BX2was Bacillus beringensis beringensis, and strain CLA-3was Cellulomonas sp..Finally, we conducted a further study on isolated bacteria. Experiments proved that thephotosynthetic bacteria Psb-2, Psb-3has a decomposition odor substances （indole and stinkyfecal hormone）.the rate of metabolism of indole and stinky fecal hormone of the strain Psb-3respectively were38.64%and66.67%. the rate of metabolism of indole and stinky fecal hormoneof the strain Psb-2respectively were64.77%and55.56%.Test to determine the BX2to expandcultivation of Bacillus culture media components: corn starch1%,、soybean flour0.5%、NaCl1%,inoculum to choose3%.Expanded combination of culture conditions: temperatures of37°c,initial PH7.2,33h training time, speed130r/min; Strain CLA-3expanded cultivation culture mediacomponents:sucrose1.9%, soybean flour0.6%, K₂HPO₄0.075%, MgSO40.075%, expandedcombination of culture conditions: temperatures of27°c, initial PH7.2,52h training time, speed110r/min.