Study On The Structure And Function Of Bacterial Consortium RAMCD407for Ramie Degumming

Ramie fibre is known as a fine plant fiber,it has the character of good toughness andbretathable and antibacterial. These features make it more suitable for textile manufacturingand rope manufacturing. Ramie degumming is a basic engineering of textilemanufacturing.The quality of hemp fiber paly a very important role on textile.The way oftraditional degumming is chemical degumming, which is scouring them with strong base.This way make a big pollution, damage fiber, and need a high cost,but ramie biologicaldegumming technology has small pollution, enhance the quality of the fiber, and need lowlabor. Therefore, as a new tecnology of degumming, there is growing emphasis on ramiemicrobial degumming. However, the biological degumming species currently used can notcomplete a degumming process in a very short time, and the degumming process need a strictenvironment, so it is difficult for us to applicate on industry.So the research need to clarify the resource status of available degummingmicroorganisms in a natural or artificial environments and optimize degumming technology, itwill help to screen efficiently degumming bacteria which can quickly applied to industry anduse rational useful genetic resources. In recent years, metagenomic technology graduallydeveloped, it can be directly study microbial genomes in the mixed flora samples. it avoidsthe problem on pure culture technology of uncultured microorganisms and open up new waysto fully utilize microbial genetic resources.The laboratory cultured by artificial bacterial consortium RAMCD407for ramiebiological degumming.By means of a new molecular biology tecnology PCR-DGGE(polymerase chain reaction-denaturing gradient gel electrophoresis) study ramiedegumming flora, taking samples every18hours,4days for a degumming cycle,extractingDNA, amplifing16SrDNA V6variable region with F968GC/R1401as primers.Thisexperiment analyzed microbial community structure of these five periods by DGGE, and theevolution of the whole flora. The results show that the type of bacterium in ramie degummingflora RAMCD407keep stable basically, some are disappearing, some are graduallyemerging.Then the dominant bands were recovered and sequenced.Then,using the Blast toolcompared sequencing results with the GenBank database which are known sequences,foundthe most similar DNA sequence and constructed phylogenetic tree. From the degumming bacterial isolated18strains by the selection medium in the brothof retting flora. By the method of Congo red hydrolysis circle and enzymatic activity, itisolated eight culturable microorganisms.Through identifying bacteria, it finds that fourbacterias is Bacillus cereus,others are bacillus subtilis, Firmicutes, pseudomonas, Bacillusthuringiensis. In the future it needs to do further study about strains unglued capacity.In this article,this experiment use DGGE technique to study the structure and function ofdegumming retting flora.Then it can learn more about the composition and evolution ofbacterial retting flora,optimize the microbial community structure, improve unglued efficiency,provide a theoretical for optimizing bacterial degumming process.