The Research Of Remediation Of Petroleum-contaminated Soil Microbial Strain Breeding In North Region Of Shaanxi

With the development of the oil industry, soil oil pollution is a growing problem. Microbial remediation method is a research focus in the current petroleum-contaminated soil remediation method, and has application prospects. The oilfield crude oil and petroleum contaminated soil of Huang Ling is the raw materials of the research, which are identified and studied on characteristic after enrichment, separation, negative screen, domestication. And to study the nutritional conditions and environmental factors that affect the degradation of performance. Provide a scientific basis for the use of layered soil column degradation of petroleum contaminants in soil to microorganisms repair petroleum contaminated soil.As varying degrees of oil pollution,the Huang.Ling oilfield in the northern Shaanxi near the soil alkaline. Oil concentration of petroleum hydrocarbon change in the159mg/kg-23582mg/kg range.29oil-degrading bacteria were isolated from petroleum-contaminated soil enrichment and separation. Rescreening domesticated and selected4. Initially identified S1and S2belonging to Plesionmonas sp., S3belonging to Pseudomonas sp., S4belonging to Zoogloea sp. S3on the oil degradation rate highest, up to56.34%.P3decline in crude oil concentration of3%solution rate can reach57.70%that indicate it has most obvious tolerance effects of high concentrations of crude. In the experiments, All of the fermentation of S1S2S3P4can produce oil spreading aim at crude oil, and mean diameters were1.03cm>.0.98cm、1.51cm、1.69cm. Efficient oil-degrading bacteria has shown rapid growth when PH at7and9. The degradation rate trend shows a drop of oil capacity and cell number of closely related. The trend is that in different initial PH value the degradation system eventually PH values are close to the neutral.4Efficient bacteria preferred NH4N03as the best N source, and drop the highest oil yield in the N P=4.4:1.Enhanced oil concentration of petroleum hydrocarbon is conducive to the growth of the degrading bacteria in it suitable for microbial life activities within, but microbial activity will be inhibited when it too high. The result that layered soil column method to study the degradation of petroleum oil pollution in soil turns out the P3was inoculated efficient degradation and added N、P nutrients can greatly promote the degradation of petroleum. The experimental group3final degreasing rate than the experimental group1average increase of nearly52%and than group2nearly25%. The effect of upper soil at the onset of the degradation initial, but in subsoil column degradation effect was significantly higher than the upper one in the late. The middle of soil falling oil of group3compared with the upper soil increased, was55.71%.