The Analysis, Preparation And Microencapsulation Of The Carotenoids From Phaffia Rhodozyma JMU-MVP14

Phaffia rhodozyma JMU-MVP14is a high-yield astaxanthin strain selected recent years. Anew HPLC method is developed to analyze the composition of carotenoids from the strainthroughout the research. The technology is established including the extraction and concentrationof carotenoids, obtaining astaxanthin crystals, microencapsulation of astaxanthin. The carotenoidkinds and contents of Phaffia rhodozyma JMU-MVP14are analyzed during the experiment. Theresults are as following:Under the chromatograph condition of a Nova-pac C18column (3.9×150mm,4μm),35oC,1.0mL/min, injection volume20μL, the proportion of organic phase (methanol, acetonitrile,tetrahydrofuran) was optimized by mixture experiment. The results showed the best proportionwas61:32:7(methanol: tetrahydrofuran: acetonitrile). The organic phase gradient was0-40minchanged from60%to100%, decreased to60%in the following5min, and maintained3min.The seperation degree of astaxanthin and other carotenoids was improved. The detection limitsof astaxanthin, β-carotene and β-cryptoxanthin respectively were0.05μg/mL,0.01μg/mL and0.37μg/mL with the new optimized HPLC method. The RSDs of astaxanthin and β-carotenerespectively were0.39%-1.15%,0.86%-2.68%；the recoveries of astaxanthin and β-carotenewere99.25%-102.10%，98.17%-102.65%, which showed good accuracy and precision.The carotenoids from Phaffia rhodozyma JMU-MVP14were analyzed by the optimizedHPLC method. The results showed it could produce many kinds of carotenoids, such asastaxanthin, β-cryptoxanthin, β-carotene and other carotenoids, and the content of astaxanthinwas the largest. Phaffia rhodozyma JMU-MVP14strains were breaking cell wall by lactic acid.It showed that the effect of breaking cell wall by lactic acid was nearly to that by DMSO, but thethe extraction rate by ethanol was bad. The carotenoids were extracted in sequence by acetone,n-hexane, ethyl acetate, ethanol, n-hexane, and the extracted ratio of carotenoids reached to78.53%. The carotenoids content of the same concentration influenced by the method ofbreaking cell wall was much different, which was less different by extraction solvent. Thecarotenoids were concentrated by rotary evaporators. The degradation of carotenoids wereinfluenced by temperature and O2, which was more remarkably to β-carotene and β-cryptoxanthin. Cryoconcentration and using N2when relieving vacuum could reduce the lossratio of carotenoids. The system of water, ether, ethanol was easier to make carotenoids crystallizing than onlyether used.18.27%of the crystals obtained by the system of water, ether, ethanol werecarotenoids, of which astaxanthin accounted for87.20%.The best wall material chosed was gelatin and starch; the optimized proportion of them was1:1; the proportion of wall material and core material was1:1; the best value of HLB was12.13(twain20:twain80:span60=1:1:1). Microcapsules were producted by spray drying under thecondition of inlet temperature190oC, outlet temperature60℃, feed rate360mL/h, hot air flow5.1m3/min, atomizer pressure0.4Mpa. The efficiency of microcapsule was61.76%, and theyield was95.29%.The composition of carotenoids from Phaffia rhodozyma JMU-MVP14were analyzed inthe research. The technical was established including the extraction and concentration ofcarotenoids and the microencapsulation. It laid the foundation of the use of carotenoids fromPhaffia rhodozyma.