Research On The Extraction Of Soy Protein And Oil In Different Reverse Micelle Systems

Reverse micelle technology is a kind of new biological separation method, and it canseparate plant oil and protein at the same time. Soybean protein has high quality and soybeanoil is a good edible oil, so this paper studied soybean protein and oil extracted from differentreverse micelles systems and provided the basis for reverse micelle extraction technology.This paper researched that AOT, AOT-Tween85, DMBAC reverse micelle systemsextracted soybean protein with ultrasonic-assisted method, and investigated single factor andorthogonal test of forward extraction and backward extraction respectively. Based on theanalysis of effect factor, the extraction mechanism was explored. The study found that AOT-Tween85system extraction efficiency was obviously higher than the other systems.It was studied that ultrasonic auxiliary AOT and AOT-Tween85reverse micelle systemsextracted soybean oil. Soybean oil extraction efficiencies of two different reverse micellesystems were different, and the forward extraction efficiency of AOT-Tween85reversemicelle systems was higher than AOT reverse micelle systems. Acid value and peroxide valueof reverse micelle extracting oil were lower than leaching, so it can be seen that oil quality ofreverse micelle extraction was better than leaching. Fatty acid composition of three methodswere same nearly.This experiment compared W0, viscosity and conductivity value of AOT/isooctane singlereverse micelle system and AOT-Tween85/isooctane-n-octyl alcohol mixed reverse micellesystem. Results showed W0of AOT-Tween85reverse micelle was bigger than AOT reversemicelle that on the same water addition, so the “pool” of AOT-Tween85reverse micellesystem was bigger, and the system could extract macromolecular protein; the viscosity ofAOT-Tween85mixed reverse micelle was greater than AOT single reverse micelle on thesame W0conditions; the viscosity of AOT reverse micelle increased and then reduced withthe increase of W0, but AOT-Tween85reverse micelle increased.Compared the structural and functional properties of soybean protein by reverse micelleextraction and alkali-solution and acid-isolation method via using Fourier transform infraredspectroscopy (FTIR), scanning electronic microscope (SEM), differential scanningcalorimetry (DSC), and X-ray diffraction, and compared the solubility and water holding capacity of different soybean protein. Through the comparative analysis it was found thatprotein structure protection of reverse micelle was superior to alkali-solution andacid-isolation method, and further testified "pool" structure of reverse micelle had a greaterprotection for protein; two kinds of methods had different shapes of protein structure;denaturation temperature of soy protein extracted by reverse micelle was higher, it showedthat the functional structure is stable relatively; solubility and water holding capacity ofsoybean protein by reverse micelle was superior than alkali-solution and acid-isolationmethod.