Compound Microbial Agents Development And Application In The Sludge Compost

Sludge compost as one of sludge treatment technology is widely used nowadays. Hightemperature fermentation can kill most pathogens while decomposition of organic matter toincrease crop fertilizer efficiency. Inoculate compound bacteria, which can regulate microbialcommunity structure in compost, faster than traditional composting of degradable materials,longer high temperature period. This study used the sludge as restrictive culture material,constructed a set of composite system F12with a strong degradation of organic matter effectunder high temperature conditions. Simulated aerobic composting, research complexmicrobial composting inoculum size and degradation characteristics. The main findings wasas follows:1. To build the sludge degradation composite microbial system. The dry sludge do onlynitrogen source in the medium, select20kinds of bacteria from environment sample or pileof manure source. Extract bacteria was inoculated in a ratio of5%, cultured with shaking at50°C,150rpm and passaged every48h with amount of5%, a total of10generations. Madethe48h sludge organic matter degradation rate in culture liquid as indicators, selected highrate of degradation of organic matter and passaged stability four kinds of composite system:C1, C4, C17, C18. Combining four strains to repeat initial screening process, organic matterdegradation rate and microbial growth curve as index, eventually sieve organic matterdegradation compound strains F12.2. Characterization of composite strains F12. Through the analysis of the growth curveand pH change curve of F12, it is found that the composite system can be faster proliferation.Changes in the pH of the fermentation process under different initial pH conditions tended tobe consistent and all can to develop in the direction of neutral, indicating that the compositestrains have a strong buffering ability to regulate the pH of the environment.3. Optimization of culture conditions. First of all single factor test to determine theappropriate range of various culture conditions. Determine the optimum pH range of themedium was6.0to8.0; preferred carbon source was glucose and sucrose; suitable carbonsource added concentration below0.5%; optimal culture inoculation amount of6%or less;optimum shaker speed range120rpm~180rpm. On the basis doing orthogonal experiment,available optimal culture conditions: glucose concentration of0.5%, the sucroseconcentration of5%, KH2PO4concentration of0.15%, MgSO4concentration of0.04%, CaCl2 concentration of0.03%, FeSO4concentration of0.02%the pH6.0, speed180rpm,5%of theamount of microbial inoculation. By variance analysis, glucose concentration, NaClconcentration, pH were the most significant impact of the whole training process. Microbialorganic matter degradation rate and cell concentration increased respectively by9.6%and9.1%under the optimized conditions.4. Sludge compost research. By different proportion, inoculated amount5%was the mostfavorable for heating up and maintain a high temperature. Vaccination can rapid degradationof organic matter, the pile body quick heating and high temperature for longer periods. Thecolonies in compost were significantly associated with temperature changes, and can belearned largest number of bacterial colonies, followed by actinomycetes and fungi in thesludge compost.At home and abroad, culture excess sludge degrading bacteria by restrictive culturetechnology are uncommon. The subject used the technology to make organic waste intoresources, has important theoretical and practical significance.