The Study Of Cell And Blood Compatibility Of Functional Graphene And Multi-Walled Carbon Nanotubes

Multi-walled carbon nanotubes and graphene, with unique structure and excellent physical and chemical properties, has a great application prospect in materials science and biomedical fields. However, graphene is easy to aggregate and difficult to disperse in the solvent and the strong hydrophobicity of multi-walled carbon nanotubes restricted their application in biomedical field. This paper, the cells and blood compatibility of material is anticipated to be improved by means of chemical doping and ion implantation on surface modification of graphene and carbon nanotubes.Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy test showed that N elements is exist by C=N valence bond in N doped graphene, N ion implantation graphene, NH2ion implanted graphene and carbon nanotubes. The contact Angle of carbon nanotubes reduced from128.97°to67.95°after NH2ion implantation, indicating NH2ion implantation can improve the hydrophilicity of carbon nanotubes. N doping, N ion implantation and NH2ion implantation make it easier for the graphene to disperse in water and organic solvents.The mouse fibroblasts and endothelial cells were selected in this article to test the compatibility of functional nanomaterials. The results show that two kinds of cells cultured on functional nanomaterials displayed higher cell-viability, proliferation, and stretching compared with those cultured on original graphene and Multi-walled carbon nanotubes. By scanning electron microscope(SEM), it can be observed that cells cultured on functional nanomaterials is flat, closely contact with materials, pseudopodia is thick and mutually intertwined, firmly fixed on material surface. Suggesting that the introduction of N elements can greatly promoted the cell compatibility of nanomaterials.Platelet adhesion and the hemolysis test were operated in this article to compare the blood compatibility of nanomaterials before and after functionalization. The number of platelet adhered on functional carbon nanotubes reduced, no reunion and activate is appeared and the hemolysis rate is lower, indicating NH2ion implantation can improve the blood compatibility of the carbon nanotubes. And the hemolysis rate of graphene, before and after functionalization, is very low, suggesting no damage to red blood cells, in addition, functional graphene can inhibit platelet adhesion and activation.