Research On The Analysis Of The Harmful Substances In Cereal Food Using Spectrophotometry

The illegal added and excess added of additive in food have become a problem that can not be ignored by China and even the world. The enhancement of the routine testing of the harmful residues in food plays a significant role in prohibiting this problem. However, most of the analyze procedures of current laboratory testing are based on the existing national standards which have tedious steps, long analysis period and toxic and harmful reagents usage etc, thus can hardly meet the demand of the market. This topic is a research on analyzing the usage of spectrophotometry to mensurate the aluminum, boron and sulfur dioxide which can provide a new standard guideline for routine laboratory sample detection.Research on the usage of spectrophotometry for aluminum in cereal food:use CAS as chromogenic agent, microwave digestion as the pretreatment method for the sample to identify the best experimental condition of chromogenic reaction. When applying this method in actual sample detection, the recovery rate is between91.2%and102.7%, the relative standard deviation is is between2.6%and5.1%, the uncertainty is18.9mg/kg (k=2)Research on the usage of spectrophotometry for boron in cereal food:use Azomethine H solution as chromogenic agent, microwave digestion as the pretreatment method for the sample to identify the best experimental condition of chromogenic reaction. When applying this method in actual sample detection, the recovery rate is between91.1%and101.6%, the relative standard deviation is between2.2%and5.3%, the uncertainty is5.46mg/kg (k=2)Research on the usage of spectrophotometry for sulfur dioxide in cereal food:compare several different kinds of non-toxic leaching reagent with the toxic leaching reagent used in national standards to identify the best experimental condition of chromogenic reaction. When applying this method in actual sample detection, the recovery rate is between95.6%and102.0%, the relative standard deviation is between2.5%and4.6%, the uncertainty is1.98mg/kg (k=2).This research established a method with simple steps, shorter analysis time and using non-toxic and harmLess reagents etc to analyze the aluminum, boron and sulfur dioxide in cereal food, thus it is easier to be promoted in the quality inspection system. And the evaluation of uncertainty further verifies that the improved method is reliable and effective and can meet the demand of the large amount of routine laboratory sample detection.