Synthesis And Property Of The Environmental Hormone17β-estradiol Molecularly Imprinted Polymers

17β-estradiol is a kind of environmental hormone, which can interfere with the normal endocrine system of human and animal body. Moreover it exists in environment widely. So the separation and enrichment of17p-estradiol has important significance.17β-estradiol molecularly imprinted polymers (MIPs) have the properties of specific absorption and selective recognization of model17(3-estradiol, but the selection of functional monomers and solvent have not been taken account during preparation process. The interactions of the environmental hormone17p-estradiol with three different functional monomers, acrylamide (AM), metharcylic acid (MAA), and methyl metharcylate (MMA), were investigated by ultraviolet (UV) spectroscopy. The solvent effect was also taken account using acetonitrile, tetrahydrofuran, and carbinol-chloroform mixture (V:V=1:1), respectively. Aided by molecular imprinting technique, three molecularly imprinted polymers and three none-imprinted polymers (NIPs) were obtained via homopolymerization, which were used for the selective recognition of17β-estradiol. The kinetic characteristics of adsorbing17β-estradiol on MIPs were also studied via static adsorption experiments. It is found that the interaction of17β-estradiol with MMA is stronger than those with AM and MAA. Among three solvents, acetonitrile is found to be the best choice for the synthesis of17β-estradiol-targeted MIPs in terms of the well-built interaction therein. The experimental results shows that MIPs derived from MMA exhibited good performance in adsorption of17β-estradiol (Qmax=46.05μmol·g-1). When the binary mixed substrates of17p-estradiol and estriol (V:V=1:1) were involved, MIPs from MMA shows highly selective recognition of17β-estradiol (40.27μmol·g-1) over estriol (16.57μmol·g-1). The adsorption kinetics data are fitted well by the pseudo second order kinetic model, while the value of R2is0.996.Meanwhile with17β-estradiol as template molecule, methyl methacrylate and hydroxy-ethyl methacrylate as functional monomers, a hydrophilic molecularly imprinted polymer was prepared via molecular imprinting technique. The binding ability of polymers to template molecules and their adsorption selectivity towards different substrates in water environment were evaluated by adsorption experiments. The kinetic characteristics of adsorbing17β-estradiol on MIPs were also studied via static adsorption experiments. The experimental results show that the maximum absorption capacity of MIPs in water is25.906μmol·g-1, while the maximum absorption capacity of NIPs is20.469μmol·g-1. The adsorption kinetics data are fitted well by the pseudo second order kinetic model, while the value of R2is0.999. High pressure Liquid Chromatography results show that the MIPs exhibited high selectivity and recognition ability to the17β-estradiol in water environment.