| The article took platycodon grandiflorum as raw material to study the best extraction method of platycodon saponins. Cold-maceration, thermal formulation, alcohol formulation, ultrasonic-water formulation were chosed to extract platycodon saponins. Took platycodon grandiflorum saponins extraction efficiency as index, uing response surface optimization to determine the optimal extraction technology. The article still studied the methods of platycodon saponins separation and purification. Respectively by means of macroporous resin D-101, glucan gel LH-twenty, glucan gel G-15through the column chromatography to separate and purify the platycodon grandiflorum crude saponins. And did the qualitative and quantitative analysis to the purification samples. The action that platycodon saponins can fall hematic fat and the inhibition for pancreatic lipase activity were studied. Draw the following conclusion:The highest extraction of platycodon saponins was got in the method of ultrasonic-water formulation extraction. Compare with the traditional return heating,it saved a lot of time, but also saved a large amount of reagent, and it can meet the platycodon saponins rapid extraction requirements.The best extraction technology conditions for platycodon grandiflorum saponins is:feed liquid ratio is1:9.75, ultrasound time is31min. ultrasound temperature is35℃.Platycodon saponins purification. Using the uv-vis spectrophotometer to scan the ginseng saponins Rgl standard sample, determined the maximum absorption wavelength is201nm.Respectively spectrum scanning the purified samples which got by the column chromatography with macroporous resin D-101, glucan gel LH-twenty, glucan gel G-15, it got the maximum absorption wavelength is199to205nm. It is so closed to the maximum absorbtion of ginseng saponin Rgl standard sample. It proved that using macroporous resin D-101. glucan gel LH-twenty and glucan gel G-15to purify platycodon crude saponins can get pure platycodon saponins.According to the vanillin-perchloric acid method to draw standard curve, using the standard curve to do purification samples quantitative analysis. When did column chromatography with macroporous resin D-101, the best purification effect was got at70%ethanol eluent. Platycodon saponins content was0.031mg/mL. Through the color reaction validated that the purification platycodon saponins was triterpenoid saponins. Used high performance liquid chromatography (HPLC) method to assay the platycodon grandiflorum saponins and platycodon grandiflorum purification samples, analyzed the corresponding spectrum and chromatogram. determined the purified sample contains platycodon grandiflorum saponins. For the research of platycodon grandiflorum saponins falling hematic fat action, choosing female health adult Wistar rats, observated the changes of mice weight and fat and hyperlipidemia rats serum lipid.The result showed:the platycodon saponins have the actionof falling hematic fat. High dose group of platycodon grandiflorum saponins can significantly reduce hyperlipidemia rats TG, TC level (P<0.01), at the same time can increase the hyperlipidemia rats hdl-c level. To LDL-C level also have a lowering effect.Through the platycodon saponins inhibition on pancreatic lipase activity in vitro experiment, took olein release rate of oleic acid as index, determined the platycodon saponins effect on pancreatic lipase activity was:when the reaction liquid platycodon grandiflorum saponins concentration was500μ g/ml, it had the largest inhibitory activity on pancreatic lipase, and the inhibition rate was42.5%. |
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