Hypolipidemic Effet Of Alkylamide From Zanthoxylum And Its Mechanism Of Action

This paper mainly evaluates the hypolipidemic effect and mechanism of Alkylamide from Zanthoxylum by in vivo experiment, instrument analysis and chemical analysis. Aim of this paper is to supply theory guide for the safety use of Alkylamide from Zanthoxylum. Conclusions of this paper are as follows:(1) We chose32female SD rats and divided into4groups randomly after1week adaptation, they are blank group, volatile oil-free group, amide group and residue group. The doses was4mg/kg body weight, and experiment period was28days. We found that body weight gain and dietary efficacy of amide group were added significantly compared with blank group. We tested TC, TG, HDL-L, LDL-L content of serum, TC, TG, fat content of liver, TC, TG, fat content, bile acid and natural sterol of feces. Blood lipids, liver lipids and fecal lipids of amide group were decreased significantly compared with other groups. The main hypolipidemic component of Zanthoxylum Oil is amide.(2) We use high fat diet to establish hyperlipidemia model.40female SD rats were divided into normal diet blank group, and high fat diet groups were blank group, low-dose group, middle-dose group and high-dose group, the fed doses were4.0mg/kg,8.0mg/kg and12.0mg/kg respectively, and experiment period was28days. Sanshoamides was extracted by organic solvent and used HPLC method to examine its purity.We found that body weight, diet intake, blood lipid, liver lipid, total cholesterol, total triglyceride of high fat diet blank group were higher than normal diet group significantly, which showed that the hyperlipidemia model was established successfully. Sanshoamides could decrease body weight gain and diet intake significantly, but has no significant effect on diet efficacy. Sanshoamides could decrease cholesterol concentration in blood, total fat, total cholesterol and total triglyceride in liver, and could decrease the injury caused by the change of fat content in liver, also it had little effect on body weight and liver weight. By the same time, sanshoamides could decrease the low-density lipoprotein and increase high-density lipoprotein significantly in blood. Sanshoamides could suppress the expression of FXR, HMG-CoA and CYP7A1mRNA in liver, but increase the expression of TRPV1mRNAin liver. Also, sanshoamides could increase the expression of ASBT and IBABP mRNA in ileum. We could concluded that the hypolipidemic effect of sanshoamides was by increase bile acid metabolism related gene in ileum and decrease cholesterol metabolism related gene in liver, thus decrease blood lipid and liver lipid to regulate cholesterol metabolism in vivo.Still, we found that there was no dose-effect relationship of hypolipidemic effect caused by sanshoamides. Low dose and high dose we chose were better than middle dose, but high dose sanshoamides would damage colon tissue. Low dose sanshoamides has not only significant hypolipidemic effect, but also no obvious damage to colon tissue.Sanshoamides could decrease blood lipid and liver lipid in high fat diet fed rats and regulate cholesterol metabolism in vivo, thus it could be further researched as hypolidemic medicine. This paper supplied theoretical basis of the hypolidemic mechanism of sanshoamides.