The Research Of Dectection And Formation Mechanism For Cholesterol Oxidation Products In Aquatic Products

Aquatic products because of its unique taste and flavor, loved by the people, but its quality and safety issues when there has been exposure. There have been reports, research and other research institutions in Europe showed that atherosclerosis and other cardiovascular diseases and long-term consumption of aquatic products related to high cholesterol oxides. This paper intends to establish a detection method aquatic products of cholesterol oxides efficient and sensitive for monitoring aquatic products of cholesterol oxides Security provides advanced technical support. At the same time research processing temperature, processing time, the oil, the impact of storage time on the aquatic processed to generate the amount of cholesterol oxides, examining these factors and their mutual relationship.Firstly, the detection of cholesterol oxides in aquatic products studied, the main results: the establishment of an accelerated solvent extraction-gas chromatography/mass spectrometry Rapid Determination of aquatic products of cholesterol oxide content-gel chromatography purification methods. With this method for determining the cholesteric enone,7(3-hydroxycholesterol,5β,6β-epoxy-based cholesterol,5α,6α-epoxy-based cholesterol,20a-hydroxy-cholesterol,3β,5α,6β-cholestane three alcohol,6-7keto cholesterol and other compounds to optimize the accelerated solvent extraction device, gel purification chromatography, gas chromatography/mass spectrometry conditions within10～1000ng/mL range, the linear correlation coefficient of up to0.9995above; good reproducibility relative standard deviation of less than7%; recovery of up to76%-103%; its high sensitivity, the method detection limit:7kinds of cholesterol oxides of0.1μg/kg or less, to meet the requirements.Then, select sea fishing for the raw materials, mainly to study the processing temperature, processing time, the oil, the impact of storage time on the aquatic processed to generate the amount of cholesterol oxides, the results showed that:the processing temperature, processing time, storage time and cholesterol a certain amount of oxide generated linear relationship; same type of oil generation amount of cholesterol oxides also have inconsistent effects.Finally, the sample material processed aquatic products, aquatic products regularly detected in the case of cholesterol oxide content. From tracking test results found that:the detection portion of cholesterol oxides in aquatic products, the main7β-hydroxycholesterol,20a-hydroxy cholesterol,5β,6β-epoxy-based cholesterol; Apart from theoretical experience, combined with aquatic products cholesterol oxide content critical control technologies were studied by chemical methods and biological methods to effectively reduce cholesterol oxide content aquatic products.