| Cholesterol oxidation products have adverse biological effects, such as cytotoxicity, mutagenicity, carclnogenicity and inhibition of cholesterrol biosynthesis. Because cholesterol oxidation products can injure aortic smooth muscle cells, lead to atherosclerosis, the determination method of cholesterol oxidation products was studied in this paper. The paper consist of four chapters. In chapter 1,the progress of determination methods of cholesterol oxidation products was reviewed, including of the sample pretreatment and method application in a variety samples, fifty references are cited. In chapter 2, a rapid and reliable method for determination of cholesterol oxidation products was investigated by gas chromatography and thin-layer chromatography . Separation ability of capillary chromatographic columns were compared, derivative reagent were elected. The separation and analysis was performed on SE-30 column with a flame ionization detector under isothermal condition. Cholesterol oxidation products are determined in whole milk powder by gas chromatography, including of extraction of lipid and sample purification. In chapter 3, cholesterol reaction with free radical-·OH in system of fenton- Haber-Weiss was studied ( PH = 7.4, 36.5℃) . In chapter 4, residual organic solvent in Nateglinide was determined by Capillary gas chromatography .
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