Surface Plasmon Resonance Imaging System For Drug Efficacy Analysis And The Development Of Gravity-induced Flow Injection Chip

Surface plasmon resonance imaging (SPRi) is an effective method for real-time monitoring of dynamic interaction. The technique has the advantages of label-free, excellently sensitive, highly selective and real-time. Thus, it shows a significant superiority in the analysis of interaction between biomolecules. Both the expandable application of SPRi in the fields of living cells detection and medical research and the miniaturization of sensor device have attracted more and more attention.In this paper, a SPRi sensor system based on the kretschmann configuration was built. Besides, a temperature control device was integrated into the system. The sensor worked as an experimental platform to achieve the real-time monitoring and extracting the dynamic information of biomolecular reactions. The final work performance reached the established standard.A novel evaluation of anti-cancer drug efficacy was proposed via surface plasmon resonance imaging platform. Specific entrapment and detection of tumor cells were accomplished by combining microfluidic technology. On this basis, the inhibitory effects of suramin and cisplatin on the proliferation of HepG2cells were investigated. By completing comparative analysis of the test results obtained by auxiliary cell counting kit-8(CCK-8) assay, we found that SPRi technology showed the potential in the field of drug research.A gravity-induced flow injection chip was manufactured for SPRi biosensors. The chip utilized the gravity force of the sample itself to realize sequential injection. After theoretical calculation, numerical simulation and parameter measurement, the results proved the gravity-induced flow injection chip had good injection and analysis capabilities. In the end, the dynamic detection of the hybridization process between complementary DNAs was achieved successfully by our SPRi system together with the microfluidic chip.In conclusion, a SPRi biosensor was developed successfully in this study. On this experimental platform, the inhibitory effects of anti-cancer drugs on the proliferation of tumor cells were investigated. The innovative method showed a prominent superiority. Besides, our sensor extracted the dynamic information of biomolecular interaction successfully when combined with the homemade gravity-induced fluid chip. The chip showed a good capability of flow injection during the experiment. The study could have positive implications for the researches of biomedical detection and sensor miniaturization.