Research On Seasonal Change Of Immune&Stress Levels Of Captive Père David’s Deer Based On Non-invasive Method

The Père David’s deer (Elaphurus davidianus) is first class protected animal in China, andin the World Conservation Union IUCN Red List as a field of extinctions, is a successful exampleof the world ’s species re-introduction project, the living status is the key fields of the study ofconservation biology and it attracted a wide spread attention. Noninvasive sampling and enzymelinked immunosorbent assay (ELISA) were used in this study to determine the indices ofphysiological stress and immunophysiology. The aim was to explore the feasible researchapproach to establish the scientific basis for the wild population’s monitoring and healthassessment in Père David’s deer. For this purpose, this study was done in dafeng milu nationalnature reserve of Jiangsu province collecting fecal sample and determine in lab during2011to2012. The main results are as follows:1．The IgA of captive Père David’s deer droppings determination: The results showed thatfecal IgA levels change from the seasonal change of view, the trend of female was does presentedfirst dropped and then picked up and the trend of male fecal IgA levels change was does acontinuous upward. By comparison of the male and female Père David’s deer fecal IgAconcentration change, both fecal IgA full average does not exist significant difference, indicatingthat the male and female Père David’s deer have the same IgA immunity.2．The IgG of captive Père David’s deer droppings assay results show that: IgG of femaledeer droppings content was down then up trend from seasonal changes, and feces of female deerwinter concentration of IgG levels in summer and autumn, a significant difference compared (p<0.05). The IgG content trends Bucks feces doe the same, but its summer, autumn and winterIgG concentration levels did not change significantly. By contrast IgG concentrations in male andfemale Père David’s deer droppings, found that IgG of female deer droppings concentration isslightly higher than the Bucks. The IgG levels of Père David’s deer droppings in July andOctober generally higher than in February, Père David’s deer anti-infective ability in summerand autumn is stronger than winter by change with the seasons.3．The IgM of captive Père David’s deer droppings determination results show that: maleand female Père David’s deer droppings from the seasonal changes, changes in IgM levelsshowed a trend down then up, and the the IgM concentration levels in the winter feces summerand autumn compared differences significant (p <0.05). The IgM levels of male Père David’sdeer droppings is higher than the female deer, and the risk of infection of male deer is higher inthe same living environment.4．Male and female Père David’s deer fecal cortisol levels and immunity levels weremeasured and found Père David’s deer fecal cortisol levels and correlation of fecal IgA, fecalIgG and fecal IgM were tested positive correlation. Description Père David’s deer was suffer adverse factor stimulation will affect the level of the immune after stress response. In addition,the occurrence of Père David’s deer stress response and immune response was synchronizationand IgG of Père David’s deer droppings is more sensitive than fecal IgA and fecal IgM asimmune physiological parameters, is the most suitable for the reintroduction of Père David’sdeer health status evaluation, detection reintroduction of Père David’s deer immunephysiological state indicators.