The Establishment Of Micropropagation Technical System Of Ailanthus Altissima

The different explants of Ailanthus altissima were used to select proper explants for tissueculture. After a series of studies, two tissue culture techniques, including direct organogenesisfrom stems with axillary buds and indirect organogenesis from callus induction, were establishedto provide an important method for asexual propogation of Ailanthus altissima.The results were as follows:1. The establishment of a sterile systemSeedling in sterile conditions. Follow the procedure: selecting healthy and plump matureseedsâ†'soaking in tap water for48hâ†'washing in gliding water for3hâ†'soaking in70%alcoholfor1minâ†'soaking in0.1%HgCl₂for13min, the aseptic seeding rate could reach to46.67%ï¼›soaking in0.5%KMnO₄for30min, the aseptic seeding rate could reach could reach to60.0%.The best sterilization method: Selecting the stem with2or3axillary budsâ†'washing ingliding water to clear concentration on the surfaceâ†'soaking in1%detergent for3minâ†'scrubingthe surface gentlyâ†'washing in gliding water between1and2hoursâ†'soakingin70%alcohol for30sâ†'soaking in0.1%HgCl₂for13min. The contamination rate could reduce to33.33%. Thegermination rate could reach to79.43%.2. Direct organogenesis from stems with axillary budsThe best time to draw materials is April to May. The best original culture medium:N₆+2.50mg·L^-16-BA+0.05mg·L^-1IBA+0.10mg·L^-1NAA. The axillary bud germination rate could reach to83.33%. The best proliferation medium: N₆+0.3mg·L^-1NAA+2.0mg·L^-16-BA.The multiplication rate could reach to3.80. The best rooting medium:1/2MS+1.0mg·L^-1NAA. The rooting rate could reach to86.67%, the average root length is3.43cm.Hardening plantlets and transplantating: Open seal membrane for21d andcover sealmembrane for2d at every turn, the survival rate of transplanting could reach to76.67%.Soakingin0.1%carbendazim for30min, selecting which the proportion of turfy soil: river sand: perliteis3:2:2as transplantatingmatrix, the survival rate of transplanting couldreach to90%. Soakingthe contaminated plantlet in0.1%carbendazim for30min and70%alcohol for20s, the survivalrate could reach to85%.3. Indirect oranogenesis from callus inductionThe best explant for callus-inducing, the tender stems of plantlet.The bestcallus-inducingmedium: N₆+1.0mg·L^-1NAA+0.15mg·L^-16-BA+0.5mg·L^-12,4-D, the inductivity could reach to100%. The best callus differentia-tion medium: N₆+2.0mg·L^-16-BA, the differentiation ratecould reach to43.3%.The best callus-rooting medium:1/2MS+1.0mg·L^-1NAA, the rooting rate could reachto60.0%. Using500mg·L^-1Vc as prevent browing agent, the browing rate could reduce to36.67%.