Establishment Propagation System Of Ailanthus Altissima Var Erythrocarpa

This experiment study at propagation system of Ailanthus altissima var. erythrocarpa.To get seeds characteristics by sow seeds. Finding appropriate concentration of IBA to promote rooting and branch of cuttings. In this experiment,the leaves of Ailanthus altissima var as explant, studied in disinfection method、types of hormones impact induction of callus tissue, differentiation, prolifetation and rooting was established.The main results are summarized as the followings:(1) Light, imbibition and GA3 effects on seed germination. The results are as followings:The seeds insensitive to illumination. Seed germination trend is consistent whether in light or dark. Seed germination rate in light is higher than dark conditions. We found no significant difference in the germination rate between seeds sucking and seeds, shows that imbibition no significant in promoting germination. When GA3 concentration of 100 mg/L have the highest germination rate reached 85.33%, can improve the seed germination.(2)The test of root and branch cuttings show that:when perennial branch in IBA concentration of 150 mg/L have the highest induction rate reached 85.33%, induction rate of annual branch can reached 60.00%. Compare With the concentration of IBA 100 mg/L no significant difference. When IBA concentration in 100 mg/L～150 mg/L, can promote the perennial and annual branches induction of callus tissue,but no root. When IBA concentration in 50 mg/L～200 mg/L, with the increase of concentration can promote survival rates、number of root, length of root. When root segment in IBA concentration of 200 mg/L have the highest induction rate reached 88.33%, highest average number of root 6.67, highest average length of root 10.50cm.(3)We establish system of tissue culture by leaves as explant. The best methods of disinfection:first the leaves rinse 5 min in running water,after soak in 30% detergent 30min and rinse 30 min in running water. The leaves were treated with 75% alcohol for 30s, sterile water for 3 times, and then 0.1% HgCl2 for 5 min, sterile water for 5 times. The optimal culture medium for callus induction of Ailanthus altissima var. erythrocarpa leaves explants was MS+6-BA 0.2mg/L+2,4-D 0.1mg/L,and the callus were yellowish-green, quality of callus relatively tight. The optimal placement was upside and induction rate reached 79.33%. After inoculation, put it on lighting environment several days and then dark, the callus were yellowish-green and induction rate reached 71.30%. The optimal culture medium for subculture callus was MS+6-BA 0.1 mg/L+2,4-D 0.1 mg/L, and callus was growing faster. The callus were quality of callus tight and yellowish-green. The optimal culture medium for the adventitious bud differentiation was MS+6-BA 3.0 mg/L+2,4-D 0.1 mg/L, and the adventitious bud was much and absinthe-green. The rooting rate reached 30% on the medium 1/2MS+IBA1.0 mg/L.