Study On The Rearing Of Long-horned Beetle Larvae(Coleoptera:Cerambycidae) Intercepted From Port And Its Instar Determination

The long-horned beetles belong to the family Cerambycidae (Coleoptera:Chrysomeloidea), with about20,000known species all the world. Long-horned beetles are important forest pests, often through the timber and wood packaging materials for long-distance communication, but also as an important medium for disseminating other pests. In recent years Port intercepted beetle species in the wood packaging and import timber increased year by year.The larvae stage of long-horned beetles is long, usually1-2years, and some species even longer, but adult stage is short, usually only a few months. Inspection and Quarantine usually only intercepted the beetle larvae that are usually difficult to identify because of their slight differences in morphological characteristics. Therefore, how to determine the beetle larval stage and how to rear larvae to adults become a bottleneck of port quarantine.In recent years, the citrus long-horned beetle, Anoplophora chinensis (Forster) is attracting wide attention in Western countries. As usual the larval stage of this species is long. Under natural conditions the larvae pupate after the winter. In this study, nine morphological structures of the larvae of citrus long-horned beetle were measured, which were further analyzed by frequency distribution, mean analysis and linear regression. The results indicated that the larval stage can be divided into five instars with fore head width as the best indicator followed by length of mandible and width of mandible.Three artificial diets were designed to rear the beetle larva. The most ideal artificial diet was selected by analyzing the survival percentage and eclosion rate of the larvae and the weights of adults. Low temperature treatment was carried out on the last-stage larva to accelerate eclosion. The results showed that the eclosion rate was the highest after being treated under5℃for30days.