| Vitamin is a kind of necessary micronutrient for fish to maintain normal life activities and growth, but VC and other heat-sensitive vitamins have suffered serious losses in feed tempering process, extrusion and post curing process. The development of liquid vitamin can make vitamin added way to spray after granulating, thus can avoid the loss of heat-sensitive vitamins in feed processing. In order to verify the feasibility of the vitamin post spraying technology, to carry out the study.Test1:Post spraying liquid vitamin (nanoscale liquid vitamin premix) compared with the traditional process for protection of the VC. The control group feed added1%solid vitamin in the mixing stage, at95℃steam curing in5minutes after granulating. The test group with95℃after granulating steam curing for5minutes, then spraying0.5‰and0.8‰, respectively, the same formula of liquid vitamin. Using fluorescence and2,4-dinitrobenzene method analysis three kind of VC content in the feed. The results showed that the0.5‰nanoscale liquid vitamin premix group and1‰solid vitamin group VC retention were same,but both lower than the0.8‰nanoscale liquid vitamin premix group.Test2:In order to evaluate the effects of the post spraying liquid vitamin and the traditional process on the growth performance of Carassius auratus gibelio, adding0.8%o and0.5‰nanoscale liquid vitamin premix to the feed for Carassius auratus gibelio with1‰solid vitamin premix of the same formula as control. Breeding trials conducted in recirculating aquaculture system. The effect on growth performance of nanoscale liquid vitamin premix with3different forms treatments with5replicates were designed for a60d trial, and each pond put an average initial weight of24.3±1.0g carp.The results showed that the weight gain ratio of0.8%o and0.5%o nanoscale liquid vitamin premix groups were higher and feed conversion ratio were lower than control while there was no significant difference between these groups.In hepatopancreas index, 1‰solid vitamin premix (5.55±0.62)>0.5‰nanoscale liquid vitamin premix (4.88±0.69)>0.8‰nanoscale liquid vitamin premix (4.77±0.42), there was no significant difference between groups (P>0.05). The same as offal index,1‰solid vitamin premix(11.71±1.07) highest,0.8‰nanoscale liquid vitamin premix (11.12±0.86) lowest.There was no significant difference (P>0.05) between groups. The fatness moderate of each group has no significant difference also. From the perspective on growth performance and physique indexes, adding0.8‰and0.5‰nanoscale liquid vitamin premix in the Carassius auratus gibelio feed and add1‰s solid vitamin premix have same effect.Test3:Study on the serum biochemical and antioxidant capacity of using liquid vitamin in feed for Carassius auratus gibelio, the experiment proposal was the same as the experiment1. The results show that in0.8‰nanoscale liquid vitamin premix group, serum TG was significantly lower than control group,while SOD activity in serum and liver, T-AOC activity in liver were significantly higher than control group. All index of0.5‰nanoscale liquid vitamin premix group had no significant difference with the control group. The results showed that antioxidant power of feeding0.8‰nanoscale liquid vitamin premix group was higher than the control group, suggesting that effectiveness of VC has been protected in liquid vitamins.Test4:Further research to investigate the effect of liquid vitamins post spraying technology. Two cages (10*10*2.5m) set in the pond of300acres, one feed was produced by post spraying technology, another was produced by traditional process. After120days of feeding, the test cage fish final weight was2980.8kg, improve268.2kg compared to the control group, the ratio raised10.9%. The test group feeding2731.2kg,228.7kg less than the control group, feed conversion ratio dropped by0.11, a decrease of5.69%. The above results prompted the liquid vitamin post spraying technology in production applications having a high feasibility. |
PDF Full Text Request