Correlative Analysis Of Microsatellite Markers And Growth Traits Among Three Populations Of Common Carp(Cyprinus Carpio)

The common Carp (Cyprinus carpio) was widely cultured and distributed in the world, and had the main position in Chinese freshwater fishery production, Of them, Jian Carp (Cyprinus carpio var. jian)、Huanghe Carp(Cyprinus carpio haematopterus Temminck et Schlegel) and Heilongjiang Carp(Cyprinus carpio haematopterus) were the important artificial and wild strains. But in the recent years, slower growth, decreased yields and lower disease resistance of common carp became a more and more obvious problem. Therefore, new strain is needed urgently in order to address these demond and the base population information which used to creat the new strain must be got. Genetic resources identification, conservation and using of such population were concerned duing to understand the strains fully. The present study analyses the diversity of Huanghe carp, Jian carp and Heilongjiang carp from the phenotype level to the molecular level, It can supply the foundation for sustainable protected such resources and genetic improvement. Further more, the correlationship between microsatellite markers diversity and the carp growth performance was also done.In this study, firstly growth traits of Jian carp, Huanghe carp and Heilongjiang carp at different ages were measured and the data was treated by correlation analysis and cluster analysis. Correlation analysis showed that Huanghe carp had a biggest correlation coefficient between body weight and body length at90days, but after150days, the correlation coefficient between body length and body depth achieved the largest. The correlations of Jian carp population in different ages between different growth traits were similar to that of Huanghe carp. But at90days, the correlation coefficient between body depth and body length of Heilongjiang carp population was0.686, lower than others. At240days, the correlation coefficients varied largely. Then, the hierarchical clustering method based on Manhattan distance was used to classify three carp populations:Huanghe carp and Heilongjiang carp belong to the same clade, and Jian carp belongs to another clade at90days and240days. On the molecular level, the genetic diversity of three carp populations was studied by using microsatellite marker technology. As a result, the highest and lowest average effective number of alleles were found in Jian carp population (3.6133) and Heilongjiang carp population (3.4533), respectively. Observed heterozygosity are also presented, the averages in each populations were0.7482、0.7643and0.7671. The average of polymorphism information content (PIC) was examined, among three populations, the Heilongjiang carp population had the highest value (0.6473) and the Huanghe carp population had the the lowest one (0.6464). So, it demonstrated that three carp populations can be used to common carp breeding program duing to the genetic diversity. The genetic distance and genetic identity study also suggested that the phylogenetic relationship between Huanghe carp population and Heilongjiang carp population was quite close, compared with Jian carp population.In order to further study the relationship of microsatellite markers above and growth traits, GLM model was established. The results suggested that Mfw5was significantly correlated with body depth in Huanghe carp (P<0.05). In Jian carp population, HIj013, Cca09, Mfw7and Mfw29were significantly correlated with body weight, body length and body depth (P<0.05); while Mfw2was significantly correlated with body weight and body width (P<0.05). In Heilongjiang carp population, Mfw6was significantly correlated with body weight, body length, body width and body depth (P<0.05); Mfw4was significantly correlated with body weight, body length and body depth; Mfw11was significantly correlated with body width and body depth. And the genotypes of these loci were determined. In our study, genotypes of specified microsatellie markers related to growth traits in these carps were determined. It may provide basis for common carp maker-assistant selection.