Preliminary Analysis On Salt Resistance And RAPD Of Different Wheat Varieties (Lines)

Wheat is the one of the main food crops in our world, and plays important role in the nationaleconomy. The restriction factor of wheat production is that salt tolerance ability is not strong in manysaline soil areas.Thirteen kinds of wheat were used as materials. In order to determine salt-tolerant ability of thirteenkinds of wheat, seed germination rate, germ length and relative salt-harm rate were researched after saltstress. At the same time, RAPD molecular marker was used to study the genetic diversity and geneticdifferentiation of nine kinds of wheat which carefully selected from thirteen kinds of wheat. The researchstudied genetic differences between different wheat varieties from morphological and molecular level. Thestudy aimed at settled a solid theoretical basis for the future use of these wheat varieties to hybrid parent’schoice、understand cross compatibility、rational utilize heterosis to breed new wheat and achieve Molecularmarker assisted breeding. The main results were as follows:1:(1) germination rate, germinal length and relative salt-harm rate of thirteen kinds of wheat wereanalyzed. The result showed that salt resistance of13kinds of wheat from high to low as follow:99110-26,Chongqing4, Jia163, Chongqing3, Xindong22, Xindong26,99034, Xindong17, Jinmai31, Yu4110, Lu23,Zhongguochun, Xindong18.(2) Thirteen kinds of wheat were divided into four categories by clusteringanalysis. The first category was strong salt-tolerant group which included99110-26and Xindong22. Thesecond category was generally salt-tolerant group which included Xindong26, Xindong17,99034, Jia163,Chongqing3and Chongqing4. The third category included Xindong18, Jinmai31and Yu4110, and theability of resistant to salt of these three varieties was poor. The third category was sensitive group whichincluded Chinese Spring and Lumai23.2: DNA extracted by CTAB method and improved CTAB method. The result showed that DNAextracted by improved CTAB method had advantages of high yield, high purity, and completely could beused for RAPD analysis.3:18random primers which could amplify clear and high repeatability bands were selected from23primers.138loci were amplified by18random primers, of which103loci had polymorphism. The averagepolymorphic rate reached up to74.64%.The result indicated that nine kinds of wheat germplasm resourceshad abundant genetic diversity at the molecular level.4: Nine kinds of jute accession could be divided into four groups and six sub-types by systemclustering. The first category was divided into two categories.1-1：99110-26and Xindong26,1-2:Xindong22. The second type was divided into two classes.2-1:99034and Xindong17,2-2：Jinmai31. Thethird kind included Chinese Spring and Xindong18, and they all the salt sensitive wheat. The fourth classincluded lumai23.(2) Nine kinds of jute accession could be divided into four groups and six sub-types byprincipal coordinate analysis based on the first and second main coordinates and combined the third maincoordinates. The first category was divided into two categories.1-1：99110-26and Xindong26，1-2：Xindong22. The second class included99034and Xindong17. The second class was not subdivided in thethird coordinate directions. This result indicated that one and two coordinate information were enough todistinguish them. The third category was divided into two subgroups.3-1：Chinese Spring,3-2：Jinmai31and Xindong18. The fourth class included lumai23. The fourth class included Lumai23. Two kinds ofclustering results were basically identical.5:12special bands and eight kinds of special absence were amplified by18random primers. Thestudy identified six varieties:99110-26, Xindong26, Jinmai31, Chinese Spring, Xindong18and Lumai23.