The Development Of SNP Marker And The Construction Of Genetic Linkage Map Of The Sea Island And Upland Cotton

It’s a fast and accurate way to use DNA molecular markers assist breeding,and it’simportant to build a saturation linkage map.The sea island cotton and upland cotton geneticmap has been built at present, such as SSR markers,which has a relatively low coverage andlocalize small number of yield and quality traits QTL and not accurate, so it′s necessary toexploit an accurate location positioning.While the development and the application of SNPmarker will undoubtedly greatly increase the density of cotton genetic map.This experiment based on screening marked primer of Xinhai21and Xinluzhong36bySSR and SNP,using the screened primers to PCR amplification the F2populations andconstructed the genetic linkage map.The main results of this study are as follows:1. The F2agronomic traits such as height and beginning section high exceed the parents,but has a lower boll rate.The individual plant growing of AL12population has broader rangethan BH12population.2.Using the assembled Shenzhen Huada gene transcription as a reference, compared thetranscript sequencing of the two previous samples with referenced gene respectively, andtested the single nucleotide polymorphism of the sample by SOAPsnp.38single-base SNPsites were selected from the prediction SNP sites, containing20sites of Xinhai21and18sites of Xinluzhong36;66pairs primers were designed according to the online tetra-primerARMS-PCR, amplify11sets primers among groups,9SNP sites.According to the law ofWendy-Haborg balance,11groups of SNP classification results can be as a molecular marker.3.This experiment chose864pairs of SSR primers to screening the two parents,79polymorphism primers were screened, as a total number of9.1%.Using the screened69pairsSSR primers and11groups SNP primers to analysis the polymorphism marker geneticlinkage of F2population,90marked sites genetic linkage map and30linkage group havebeen built.The average distance between markers was55.9cM, and the total length was3135.84cM, covered62.71%of the cotton genome.