| Rehmannia glutinosa, one of the most famous medicinal plants, is a perennial herbaceous plant of theScrophulariacea family, the root tuber of Rehmannia glutinosa always used as traditional Chinese medicine.Rehmannia glutinosa was planted in Henan, Shanxi, Shanxi, Shandong and other places, but thegeo-authentic producing areas is “huaiqing prefecture” in Jiaozuo, and also has the longest plant historythere. Rehmannia glutinosa is one of the famous “four huai medicine”. Many cultivars or varieties wereselected and cultivated in China in a long history of Rehmannia glutinosa cultivation practices. Thesecultivars and varieties have many differences in the external form, internal structure, yield and quality.On the basis of previous study, we established a core germplasm repository include eight majorvarieties of Rehmannia glutinosa, evaluated them on the external form, internal structure, the chemicalmarker components and chemical fingerprint, discusses the basis to divide the germplasm and the methodof Traditional Chinese Medicine Fingerprint(TCMF) to identify the quality of Rehmannia glutinosa. Wefocus on the yield and quality to the same cultivar of Rehmannia glutinosa which called Beijing No.3indifferent areas, to find out the best place to expand the plant area. With the eight cultivars of the germplasmrepository, we designed the hybrid experiment by randomized complete block design, to discussrelationship between the genetic relationship of hybridized combination, This test regulated the standard ofRehmannia glutinosa hybridization technique, and the creation of61hybrid offspring laid the foundationfor creating new germplasm and breeding new varieties of Rehmannia glutinosa.1. Using ruler measurement and scanning electron microscopy (SEM) method compared the leafmorphological characteristics of eight Rehmannia glutinosa. Different varieties of Rehmannia glutinosadivide large and small leaves according to the leaf length, width and breadth. According to the density ofleaf stomatal apparatus in both upper and lower surface can be divided into high density, medium densityand low density. Classify dense hair type and few hair types according to skin the nonglandular hairydensity. Different germplasms are different in the length of the nonglandular hairy, size of the stomatalapparatus and density vary, all of these can be used as a basis for the division of Rehmannia glutinosagermplasms. 2. Using scanning electron microscopy (SEM) method compared the pollen grain shapes, germ pore,depth and density of surface ornamentation. Then, find out that all of pollens have the oval shape and threeapertures. Different varieties of Rehmannia glutinosa divide large, middle and small type according to thepollen size. According to the density of the surface ornamentation can be divided into high density,medium density and low density. All of these can be used as a basis for the division of Rehmanniaglutinosa germplasms.3. Determined the content of catalpa and acteoside in eight varieties of Rehmannia glutinosa viahigh-performance liquid chromatographic method. Found that there is a difference in the content of catalpaand acteoside of different Rehmannia glutinosa germplasms. Contents of catalpa: Qinhuai (3.70%)（CK）﹥HuaidiNo.3(3.68%)（P＞0.05）﹥85-5(3.05%)(P＜0.01)﹥Shengjin (2.91%)(P＜0.01)﹥Beijing No.3(2.55%)(P＜0.01)﹥HuaidiNo.2(2.31%)(P＜0.01)﹥Virus-free85-5(2.30%)(P＜0.01)﹥Beijing No.1(2.25%)(P＜0.01)﹥Huaidi No.1(2.23%)(P＜0.01); Contents of verbascoside:85-5(0.13%)（CK）﹥Beijing No.1(0.12%)（P＞0.05）﹥HuaidiNo.2(0.10%)(P＜0.01)﹥Shengjon (0.10%)(P＜0.01)﹥HuaidiNo.3(0.08%)(P＜0.01)﹥HuaidiNo.1(0.06%)(P＜0.01)﹥Qinhuai (0.04%)(P＜0.01)﹥Virus-free85-5(0.04%)(P＜0.01)﹥Beijing No.3(0.03%)(P＜0.01).4. We optimized HPLC technology conditions by high performance liquid chromatography. Thestandard fingerprint chromatogram for Henan trueborn Rehmannia glutinosa was built which has20characteristic peaks be determined. Similarity and cluster analysis was taken in different varieties by HPLCfingerprint, and provided that it is a reliable method for variety identification and quality control ofRehmannia glutinosa.5. Beijing No.1was planted in Wenxian, Huojia, Xinxiang-beihuan, Fengqiu, Shanxian, the yield ofthe same variety has no significant difference in this comparative experiment. We can see the fiveproducing areas are suitable for planting Rehmannia glutinosa. From marker components, we can seeRehmannia glutinosa planted in these five ateas comply with Chinese Pharmacopeia, but not better than thegeo-authentic producing areas in quality. Comprehensive each index points out that from Wenxian to theeast along Yellow River alluvial plain via Huojia, Xinxiang-beihuan, Fengqiu, and Shandong-Shanxian aresuitable for planting Rehmannia glutinosa. And the development of Rehmannia glutinosa plant accordingto the local conditions. 6. Using eight Rehmannia glutinosa cultivars in the germplasm repository and a wild cultivar, hybridexperiment was carried out by randomized complete block design. There were a total of72hybridcombinations, and the fruits rate of hybrid combination, the average seed number of single fruit, the1000-seed weight, the relationship between the average seed number of single fruit and the1000seedweight of obverse (SR) and reverse(RS) crosses are explored. The results showed that the farther hybridgenetic relationship of cultivars, the higher of the fruit rate, the seed number of single fruit and the1000-seed weight were also relatively higher, there was a certain difference between obverse (SR) andreverse(RS) crosses. Intravarietal crossing cannot bear seeds. This experiment created61hybrid offspring,and laid the technique and foundation for breeding new varieties of Rehmannia glutinosa. |
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