Gene CG7510and Radix Acanthopanax Senticosus Extract On Immune Function Of Drosophila

The innate immune system is very important defense against infection. In this study, the main experimental material is the Drosophila melanogaster. To study the function and mechanism of CG7510in the innate immune system of D. melanogaster. Rats were immunized with purified proteins of CG7510to produce polyclonal antibody. The distribution of CG7510was observed using fluorescence microscopy after immunostaining blood cells of D. melanogaster. The expression of CG7510in different stage and tissue were detected by semi-quantitative PCR. The expression of antimicrobial peptides and signaling pathways factor were detected by semi-quantitative PCR too. The protein of CG7510is the membrane protein. The expression of CG7510decresecd and then increased in develop processes. The expression of CG7510were the lowest level in first instar larval stage. The study showed that CG7510can affect the expression of CeCA2and Dfn. The discovers were provided important theoretical basis for the study on the funtion of CG7510in innate immune.Intestinal immunity is a complex system. To study the effect of the extract from the roots and rhizomes of Acanthopanax senticosus (ERA) and extract especially here from the fruits of of A. senticosus (EFA) on innate immunity and gut immunity of D. melanogaster. D. melanogaster was infected by bacterial or fungal biological solution with or without ERA or EFA, and the effects of ERA or EFA on the survival rates of D. melanogaster w ith microbial infection and thermal stimulation were recorded. Cell apoptosis was observed using fluorescence microscopy after immunostaining gut tissue of D. melanogaster. The total RNA of spore-infected D. melanogaster was extracted and the expression of antimicrobial peptides was detected. ERA and EFA could improve the survival rate of bacteria-infected D. melanogaster, meanwhile alleviate the apoptosis of gut epithelial cells. The survival rate of spore-infected D.melanogaster was improved by ERA and EFA, and certain antimicrobial peptides were highly expressed in vivo. ERA and EFA could also improve the survival rate of D. melanogaster with thermal stimulation. ERA and EFA could significantly improve the immune function of D. melanogaster.