Application Of Nitrogen-Fixing Bacillus Megaterium On The Cultivation Of Eucalyptus

The nitrogen-fixing Bacillus megaterium are one of the probiotics species, possessing high ability of nitrogen fixation. In this study, the bacterial manure was prepared with the pre-screening Bacillus megaterium DU07, and the eucalyptus afforestation trial proceeded with the randomized block experimental group design. The test ground is located at the National Forest Park Hill District in Liangfengjiang, Guangxi province. The growth of Eucalyptus was measured after afforestation of2,6,12and16months; the eucalyptus rhizosphere microorganisms, soil enzyme (protease, urease, invertase, peroxidaseand catalase) activity and nutritional elements (including total nitrogen and nitrogen) were determined at the forest age of9,12,15and18months, exploring its role on the growth of Eucalyptus and providing a reliable basis for the scale of the development and application.The conclusions were shown below:(1) The growth of Eucalyptus reach the highest in the eucalypt-special-fertilizer (CESF) group, and followed by the bacterial manure-fertilized group, the lowest was self-made inoculated fertilizer group; In addition, the application of bacterial manure could increase the tree height and diameter at breast height, has a certain role in promoting the growth of eucalyptus. (2) The bacterial manure made of the strains cultured in different mediums affected on the Eucalyptus without significance, but some differences occurred on the Eucalyptus DBH (diameter at ground). It was also found that the bacterial manure made by the substrate of cassava dregs fermentation or peat soil acted on the growth of Eucalyptus without significance.(3) The quantity of Rhizosphere soil microbes (including bacteria, fungi and nitrogen-fixing bacteria) in the bacterial manure-fertilized group was higher than the self-unvaccinated-fertilized group. While the number of actinomycetes of the eucalyptus rhizosphere bacteria in the bacterial manure-fertilized group was the lowest in the three groups after18months. In addition, bacteria held the largest number and followed by nitrogen-fixing bacteria, fungal again, and the lowest number was for actinomycetes, indicating that the application of nitrogen-fixing bacteria manure could improve the biological activity of the soil.(4)The number of colonies of eucalyptus rhizosphere bacteria in each group does not meet the significant difference in different forest age. Fertilizer in the matrix with the same strains while cultured in different media could lead to the number of colonies of eucalyptus rhizosphere bacteria in each group in different age did not reach significant difference. Eucalyptus rhizosphere soil enzymes (protease, urease, invertase, peroxidase and peroxide and catalase) activity of the bacterial manure-fertilized group was higher than the self-made inoculated fertilizer group, and along with the dressing process, the difference of the enzyme activities also increased.(5) Eucalyptus soil total nitrogen and nitrogen content in the highest content in the bacterial manure-fertilized group, and followed by the Eucalyptus-special-fertilizer group, finally with the unvaccinated strain fertilizer group. In the configuration of the bacterial manure, the difference with its matrix (fermented cassava residue and peat) and strains medium (beef extract, peptone and A Sugai medium) acted on the eucalyptus soil enzymes and nutritional elements slightly.