Study On Genetic Diversity Of Liriodendron Chinense

This paper studied on genetic diversity of Liriodendron chinense by ISSR makers, sampled from6natural populations (Anhe, Quanzhou; Yachang, Leye; Liangshui, Ziyuan; Minglun, Huanjiang; Antai, Rongshui; Laobao, Rongshui) in Guguangxi University and9provenances test samples (Bucheng, Huan; Xiuning, Anhui; Lu moutain, Jiangxi; Lichuan, Hubei; Wuyi moutain, Fujian; Quanzhou, Guangxi; Liping, Guizhou; Jinping, Yunnan; Anji, Zhejiang) in Xianshui Forest Farm (Quanzhou Guangxi) and Gaofeng Forest Farm (Nanning Guangxi). The main conclusions showed as follows:(1) From the comparison of quality, purity, agarose gel electrophoresis results, stability etc.The DNA extraction of Liriodendron chinense result showed that the Plant Genomic DNA Extraction Kit method was batter than the improved CTAB method.(2) The establishment of optimal reaction system was:20μL reaction volume containing2.0μL10×buffer,1.8mmol/L MgCl2,0.2mmol/L dNTPs0.5μmol/L primer,1U Taq polymerase,60ng template DNA. The final PCR amplification program was:94℃for5min,94℃for1min, N℃for45s,72℃for2min,45cycles,72℃for7min.(3) Primer Screening was:UBC-807, UBC-809, UBC-816, UBC-817, UBC-818, UBC-825, UBC-826, UBC-829, UBC-839, UBC-847, UBC-849, UBC-850, UBC-851, UBC-854, UBC-878, UBC-880, UBC-881, UBC-887, UBC-889, UBC-890, UBC-891. Which can be Amplified. Elected10best primers which were used in final ISSR-PCR from the above primers. They were UBC-807, UBC-818, UBC-825, UBC-826, UBC-829, UBC-847, UBC-881, UBC-889, UBC-890, UBC-891.(4) The analysis of6Natural populations (Anhe, Quanzhou; Yachang, Leye; Liangshui, Ziyuan; Minglun, Huanjiang; Antai, Rongshui; Laobao, Rongshui) was:10primers were Amplified126legible bands, in which123bands were polymorphic with. The PPB was97.62%, the highest Natural population was Antai, Rongshui. The total genetic variation in28.95%among the populations,71.05%within the populations. According to the UPGMA cluster analysis, Natural populations were divided into two embranchments, one was Minglun, Huanjiang;Antai, Rongshu and Yachang, Leye.the other one was Anhe, Quanzhou; Laobao, Rongshui and Liangshui, Ziyuan.(5) The analysis of9Provenances test samples (Bucheng, Huan; Xiuning, Anhui; Lu moutain, Jiangxi; Lichuan, Hubei; Wuyi moutain, Fujian; Quanzhou, Guangxi; Liping, Guizhou; Jinping, Yunnan; Anji, Zhejiang) was:10primers were Amplified126legible bands, in which123bands were polymorphic with. The PPB was97.62%, the highest Provenances test samples was Lichuan, Hubei. The total genetic variation in25.37%among the populations,74.63%within the populations.According to the UPGMA cluster analysis, Provenances test samples were divided into northern and southern two embranchments on the whole, and the southern region were Subdivided into western region,the central region and eastern region three embranchments.(5) By analyzed genetic diversity of natural populations and provenance test samples, this paper proposed the strategies and measures of germplasm conservation.