Turn Cry1Ab Insect-resistant Genes Of Maize Of Molecular Biology Research

Maize is the most important food, feedstuff and energy crop in the world. With the continueddevelopment of world economy and the growing of population, the demand for maize is in a growing trendin China. But with the worsening of environment, plant diseases and insect pests of maize is much heavier.According to the FAO statistics, losses caused by pests of maize occupies12%each year in the world.Therefore, improve the production of maize, breeding various kinds of disease-resistant, insect-resistantmaize is becoming urgent. At present, the breeding of new varieties of crops have been gradually shiftedfrom the traditional breeding to the breeding by using transgenic technology. Since the first commercialcultivation of genetically modified crops achieved success, the area of genetically modified crops have keptgrowing and reached134million hectares in2009.This paper mediated by gene gun law will Cry1Ab insect-resistant gene into Hi â…¡A maize callus, getthe transgenic Cry1Ab positive maize seedling. By comparing expression quantity of the transgenic Cry1Abmaize at the transcriptional level, the paper discusses the the relationship between insect resistance indifferent organization parts and the biological characteristics of maize. By transform worm to thetransgenic Cry1Ab maize, it also explores the effectiveness of the Cry1Ab protein expression, and providesreference for insect resistance Cry1Ab gene research. Cry1Ab gene maize plants through the investigationand analysis of agronomic characters, the paper discusses the changes of genetically modified maize andcontrol of maize plant production, provides basis for breeding work of the genetically modified maize.1, By the particle bombardment method, artificial modified Cry1Ab gene was successfullytransformed into maize callus, PCR positive seedling was9strains, Bt protein expression positive seedling5plants, T0generation of genetically obtain3strains.2, According to the PCR and immune strip test to the maize inbred lines of generation T1to T3, weeventually got stable genetic Cry1Ab gene maize plants.3, by RT-PCR and fluorescence quantitative PCR to turn Cry1Ab gene maize each histologic sectionsof the stable genetic expression at the transcriptional level testing, results showed that the leaf of Cry1Abamount of expression is significantly higher than leaf sheath and the roots.4, The effectiveness of testing the objective traits by putting insects on maizes further provided proofsthat genes were transformed into maize plants, and the target protein were all effectively expressed. 5, Agronomic traits in the field surveys showed that when comparing with the comparison maizes, thetransgenic Cry1Ab gene showed a trend of increase.