Study On Impact Of Cry1Ab/c Transgenic Insect-Resistant Rice On Soil Enzyme Activities And Rhizospheric Microorganism

The consistent ecological, economical and social benefits were generated from transgenic crops. However, people concerned about the ecological risk associated with transgenic crops. In this paper, we used transgenic Bt insect-resistant rice of Shan you63and transgenic Bt insect-resistant rice of Hua hui1which were issued the safety certificate to evaluate the impact of transgenic rice and its parental non transgenic control on soil enzyme activities and rhizosphere soil microorganism combining traditional pure culture techniques with modern molecular means. The results are as follows:Firstly, the planting of transgenic Bt insect-resistant rice of Shan you63and transgenic Bt insect-resistant rice of Hua hui1failed to show any distinct difference in the quantity of microorganism including fungi, bacteria, actinomycetes between transgenic crylAb/c and its non transgenic parental control. Instead, there were some significant differences during the rice growth stages.Secondly, compared with its parental non transgenic control, the fungal communities of transgenic Bt insect-resistant rice of Shan you63were the same, but the dominant parts were smaller than it, the part is Hypocrea; The bacteria communities of transgenic Bt insect-resistant rice of Shan you63were fewer than it, the parts were Staphylococcus and Rhodococcus, but the dominant parts were the same; Both the actinomyces communities and the dominant parts of transgenic Bt insect-resistant rice of Shan you63were the same.Compared with its parental non transgenic control, the fungal communities of transgenic Bt insect-resistant rice of Hua hui1were the same, but the dominant parts were smaller than it, the part is Hypocrea; The bacteria communities of transgenic Bt insect-resistant rice of Hua hui1were fewer than it, the part was Staphylococcus, but the dominant parts were the same; Both the actinomyces communities and the dominant parts of transgenic Bt insect-resistant rice of Hua hui1were the same.Thirdly, the planting of transgenic Bt insect-resistant rice of Shan you63and transgenic Bt insect-resistant rice of Hua hui1was found no significant difference in the soil nutrients including pH value, organic matter, available P, available K and available nitrogen between transgenic cry1Ab/c and its non transgenic parental control. Instead, there were some significant differences during the rice growth stages. Fourthly, whether transgenic Bt insect-resistant rice of Shan you63or transgenic Bt insect-resistant rice of Hua hui1failed to show any difference in the soil enzyme activity including invertase, acid phosphatase and alkaline phosphatase between transgenic crylAb/c and its non transgenic parental control. Instead, there were some significant differences during the rice growth stages. Some significant differences were found in the soil urease activity between transgenic Bt insect-resistant rice of Hua hui land its non transgenic parental control, the soil urease activity of Hua hui1is obviously inhibited, but no difference was found in the soil urease activity between transgenic Bt insect-resistant rice of Shan you63and its non transgenic parental control.Finally, by sequencing the clone of the16SrDNA library and18SrDNA library and analyzing the sequence using MEGA5.0software, no difference was found in the main bacteria communities’composition between transgenic crylAb/c and its non transgenic parental control, the minority exits. Some significant differences were found in the fungal community composition.