Effects Of Diets On The Larval And Adult Intestinal Bacterial Communities Of Dastarcus Helophoroides

Dastarcus helophoroides (Fairmaire)(Coleoptera: Bothrideridae) is one of the mosteffective natural enemies against the larvae and pupae of various trunk borers, such asAnoplophora glabripennis Motschulsky, Monochamus alternatus Hope, Mallambyx radeiBlessig, Apreona swainsoni (Hope), Batocera horsfieldi Hope and Apriona germari (Hope).Because the narrow distribution and low density of D. helophoroides in nature limit itsfunction in biology control, it is necessary to develop artificial feeds for D. helophoroides. Itis likely to have an effect in intestinal bacterial communities according to using artificial dietsfor D. helophoroides larvae and adults, and then affecting the use of nutrition. The diversityof the intestinal bacterial communities in Dastarcus helophoroides larvae and adults wasassayed by PCR-DGGE to determine whether different artificial diets could influence thesebacterial communities. Two diets were used for feeding the larvae and four for the adults. Theresults showed:1Bacillus, Staphylococcus, Planococcaceae, Ralstonia, Acinetobacter and Pseudomonaswere isolated from the gut of the larvae fed Diet V. Escherichia, Bacillus, Staphylococcus,Kurthia, Ralstonia, Leptothrix, Acinetobacter and Pseudomonas were isolated from the gut ofthe larvae fed Diet VI. Planococcaceae was only isolated from Diet V. Escherichia, Kurthia,and Leptothrix were isolated from Diet VI.2Desemzia, Staphylococcus, Asticcacaulis, Aurantimonas and Escherichia were isolatedfrom the gut of the adults fed Diet I; Escherichia, Desemzia, Staphylococcus, Asticcacaulis,Cellvibrio, Aurantimonas and Planomicrobium were isolated from the gut of the adults fedDiet II; Escherichia, Desemzia, Staphylococcus, Asticcacaulis, and Cellvibrio were isolatedfrom the gut of the adults fed Diet III; Escherichia, Desemzia, Staphylococcus, Asticcacaulis,and Cellvibrio were isolated from the gut of the adults fed Diet IV. Aurantimonas was onlyisolated from Diet I and Diet II; Planomicrobium was only isolated from Diet II; Cellvibriowas not isolated from Diet II.3The quantity of intestinal bacteria from the larvae fed the darkling beetle pupae wasgreater than those fed the artificial diet. The amount of intestinal bacteria from the adults feddifferent diets had the following ranking: ant powder as the major component> darkling beetle pupa powder as the major component> cricket powder as the major component>silkworm pupa powder as the major component.4In two diets for the larvae, the diversity of intestinal bacteria from the larvae fed thedarkling beetle pupae (HDiet V=2.597) was less than those fed the artificial diet (HDiet VI=2.731). In four diets for the adults, ant powder as the major component and silkworm pupapowder as the major component showed the highest (HDiet IV=1.905) and the poorest (HDiet I=1.634) biodiversity, respectively. Cricket powder as the major component and darklingbeetle pupa powder as the major component, Shannon index (H) were equaled to1.799and1.824, respectively.Conclustion: There were some differences in intestinal bacterial communities betweenDiet V and Diet VI. The identical intestinal bacterial communtites were found form Diet IIIand Diet IV, so they had identical effect of nutrition. The intestinal bacterial communities fedDiet II. Therefore, Different diets have different effect in the larvae and adults intestinalbacterial communities of D. helophoroides.