Evaluation Of Main Cultivated Species Of Pleurotus Spp.Germplasm Resources In Guangxi

Mushroom production has developed rapidly in Guangxi these years, Pleurotus was the main cultivated species of Guangxi, the scale of cultivation expanded continuously. However strains in messy management, random named and synonyms, this lead to increasing cost and difficulty in spawn management. Based on the above reasons, we carried out this research. In this study,15main cultivars strains in Guangxi selected as tested strains, excluding synonyms, doing classification and genetic diversity by antagonistic reaction, RAPD molecular marker, ITS sequence analysis,28S rDNA D1/D2region analysis and morphism of fruiting bodies.1. Pleurotus could be divided into5groups according to the results of antagonistic reaction in15strains, first group contains Zaoqiu65, Xiping1, Heipingwang, P015, P012; second one inclues Huimei2, Gaochan8105, Tekang650; Xiuzhen0811and Xiuzhen0910were classed in third group; Xingbaogu was classified in fourth group; the last one contains Ping214, Jigu1007, Jigu33, Jigu96; strains with no antagonism may be synonyms.2. Choosing six primers for RAPD markers, primer S62and S78can get polymorphism amplification, According to the cluster analysis, the strains can be divided into five groups at genetic similarity of0.75, according to dendrogram and electrophoric patterns analysis, the results was in accordance with that by antagonistic reaction RAPD indicated that bands were similar in each strains with no antagonism, and the bands were obviously difference in strains with antagonistic reaction.3. Sequencing the ITS region of tested16strains and comparing with the data from NCBI, five strains were Pleurotus ostreatus, two strains were P. pulmonarius, three strains were P. pulmonarius var., one strains was P. eryngii, four strains were P. florida, one strain was Hypsizygus marmoreus.4. By analysising the base pair distribution of ITS sequences of5strains with antagonism and5strains with no antagonism,(G+C)%is smaller than (A+T)%,(G+C)%is about44%;5.8S was conserved sequence is of154bp,5.8S of the strains was same; ITS1and ITS2change rapidly in evolution, which was250bp long; sequence variation in strains with antagonism, no sequence variation with no antagonism.5. Doing the amplification and analysition of28S rDNA D1/D2area of15strains of Pleurotus spp, D1/D2region of every strains was conserved sequence and cannot be used to identify Pleurotus spp.6. Temperature test showed that the optimum growth temperature of4strains with antagonistic reaction is30℃, mycelial growth rate can be11.60-14.29mm/d;40℃can lead strains to death.7. The cultivating experiments showed that the fruit body were similar among strains with no antagonism, thus the strains with antagonism have significant difference.