Researches On The Application Of Anti-Apoptotic Reagents To The Cryopreservation Of Boar Semen

The objective of the present study was to further improve the quality of frozen-thawed boar semen and to provide reference data for the application of anti-apoptotic reagents to the cryopreservation of boar semen. Fresh semen was collected from adult boars with the gloved-hand technique. And immediately after pretreatment, samples were diluted in an extender solution supplemented with different doses of anti-apoptotic drugs-melatonin (0,1,5,10mmol/L), dopamine hydrochloride (0,0.1,1,10mmol/L), puerarin (0,0.1,0.01,0.001mmol/L), ligustrazine hydrochloride (0,0.08,0.1,0.12mmol/L), metoprolol tartaric acid (0,1,0.1,0.01mmol/L), and dichloroacetate (0,1,0.1,0.01mmol/L). Then extended semen was frozen and thawed before spermatozoa were stained with Coomassie brilliant blue. And finally, the deformity and acrosomal integrity of spermatozoa were assessed with a microscope. The results show that (1) among three concentrations of melatonin added to freezing diluents, only10mmol/L of melatonin significantly improved the deformity and acrosomal integrity of frozen-thawed boar spermatozoa (2) under the conditions of this experiment, three doses of dopamine hydrochloride significantly enhanced the abnormality and acrosomal integrity of frozen-thawed boar spermatozoa. From the economical and mass-production point of view, the best concentration of dopamine hydrochloride was0.1mmol/L (3) different concentrations of ligustrazine hydrochloride added to boar semen freezing extenders, of which0.12mmol/L of ligustrazine hydrochloride was the best, significantly reduced the deformity or increased the acrosomal integrity of frozen-thawed spermatozoa (4) addition of a certain amount of puerarin to freezing diluents neither significantly reduced the deformity nor advanced the acrosomal integrity of frozen-thawed boar spermatozoa (5) supplementation of dichloroacetate to freezing solutions didn’t demonstrate significant improvement of the deformity or acrosomal integrity of frozen-thawed boar spermatozoa and (6)there wasn’t significant effect of metoprolol tartaric acidon on the quality of frozen-thawed boar semen.